Document Detail


Properties and fluctuations in vivo of rat liver antizyme inhibitor.
MedLine Citation:
PMID:  2730588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antizyme inhibitor was highly purified from rat liver by using affinity chromatography. It has some structural resemblance to ornithine decarboxylase (ODC), as judged from Mr, immunoreactivity and reversible binding with antizyme. However, unlike hepatic amounts of ODC and ODC-antizyme complex, that of antizyme inhibitor did not show much fluctuation upon putrescine treatment, whereas it decreased as rapidly as ODC decay in the presence of cycloheximide. These results suggested that antizyme inhibitor is an independent regulatory protein rather than a derivative of ODC. Changes in hepatic amounts of antizyme inhibitor, antizyme and ODC upon feeding suggested that antizyme inhibitor may play a role in ODC regulation by trapping antizyme and thereby suppressing ODC degradation. A monoclonal antibody to rat liver antizyme inhibitor was obtained. This antibody was shown to be utilizable for a simple assay of antizyme-inhibitor activity in tissue extracts.
Authors:
Y Murakami; S Matsufuji; M Nishiyama; S Hayashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  259     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-07-03     Completed Date:  1989-07-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  839-45     Citation Subset:  IM    
Affiliation:
Department of Nutrition, Jikei University School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Carrier Proteins*
Liver / analysis*
Ornithine Decarboxylase / antagonists & inhibitors
Proteins / analysis,  antagonists & inhibitors,  physiology*
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Carrier Proteins; 0/Proteins; 0/ornithine decarboxylase antizyme; 0/ornithine decarboxylase antizyme inhibitor; EC 4.1.1.17/Ornithine Decarboxylase
Comments/Corrections

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