Document Detail


Properties of an equine herpesvirus 1 mutant devoid of the internal inverted repeat sequence of the genomic short region.
MedLine Citation:
PMID:  21176938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 150 kbp genome of equine herpesvirus-1 (EHV-1) is composed of a unique long (UL) region and a unique short (Us) segment, which is flanked by identical internal and terminal repeat (IR and TR) sequences of 12.7 kbp. We constructed an EHV-1 lacking the entire IR (vL11ΔIR) and showed that the IR is dispensable for EHV-1 replication but that the vL11ΔIR exhibits a smaller plaque size and delayed growth kinetics. Western blot analyses of cells infected with vL11ΔIR showed that the synthesis of viral proteins encoded by the immediate-early, early, and late genes was reduced at immediate-early and early times, but by late stages of replication reached wild type levels. Intranasal infection of CBA mice revealed that the vL11ΔIR was significantly attenuated as mice infected with the vL11ΔIR showed a reduced lung viral titer and greater ability to survive infection compared to mice infected with parental or revertant virus.
Authors:
ByungChul Ahn; Yunfei Zhang; Nikolaus Osterrieder; Dennis J O'Callaghan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-12-21
Journal Detail:
Title:  Virology     Volume:  410     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-28     Completed Date:  2011-03-09     Revised Date:  2013-12-10    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  327-35     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
DNA, Viral / genetics*
Disease Models, Animal
Female
Herpesviridae Infections / pathology,  veterinary
Herpesvirus 1, Equid / genetics,  growth & development,  pathogenicity,  physiology*
Inverted Repeat Sequences*
Lung / virology
Mice
Mice, Inbred CBA
Sequence Deletion*
Survival Analysis
Viral Plaque Assay
Viral Proteins / biosynthesis
Virulence
Virus Replication*
Grant Support
ID/Acronym/Agency:
AI-22001/AI/NIAID NIH HHS; P20 RR018724/RR/NCRR NIH HHS; P20 RR018724-07/RR/NCRR NIH HHS; P20 RR018724-08/RR/NCRR NIH HHS; P20-RR018724/RR/NCRR NIH HHS; R01 AI022001/AI/NIAID NIH HHS; R01 AI022001-22/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Viral Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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