Document Detail

Properties of cell lines established from transitional cell cancers of the human urinary tract.
MedLine Citation:
PMID:  66978     Owner:  NLM     Status:  MEDLINE    
Characterization studies have been carried out on eight cell lines (253J, 192B, 639V, 647V, 486P, 575A,743E, and 751G) established from transitional cell cancers of the human urinary tract. Although subtle morphological differences exist among individual lines, each has an epithelial morphology and exhibits multilayering. The doubling times for the cells range from 20 to 56 hr, and at least a 1-to-3 split can be achieved when they are subcultured every 4th day. Karyotypic analysis revealed a hyperdiploid stemline for each cell line, and presence of a Y chromosome was confirmed by Q banding in five of the lines. The tumorigenic nature of the cell lines was demonstrated by their production of tumors in hamsters and confirmed by colony formation in agar. The transitional cell cancer lines were shown to be free of Mycoplasma, and their glucose-6-phosphate dehydrogenase mobility patterns and their Karyotypes prove that they are not HeLa cells.
A Y Elliott; D L Bronson; J Cervenka; N Stein; E E Fraley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  37     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-06-22     Completed Date:  1977-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1279-89     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Transitional Cell / pathology*
Cell Division
Cell Line*
Hela Cells
Neoplasms, Experimental / etiology
Sex Chromosomes
Staining and Labeling
Urogenital Neoplasms / genetics,  pathology*

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