Document Detail

Properties of cadmium-binding proteins in rat testes. Characteristics unlike metallothionein.
MedLine Citation:
PMID:  4062897     Owner:  NLM     Status:  MEDLINE    
Since the exposure of rats to cadmium causes zinc to accumulate in metallothionein in liver and kidney but not in a similar protein in the testes, the properties of the low-Mr cadmium-binding proteins were investigated in rat testes. Weanling rats that had been given dietary cadmium for 6 weeks were injected with 109CdCl2 and subsequently killed, and the 109Cd-labelled low-Mr proteins from testes were purified. The pooled low-Mr cadmium-containing fractions from the gel-filtration (Sephadex G-75) columns were eluted through DEAE-Sephacel columns, yielding two peaks. Each of the individual peaks from this Sephacel column was further purified by rechromatography on DEAE-Sephacel and on Bio-Gel P-10 columns. Amino acid analysis of the two purified proteins revealed a low cysteine (about 3%) content, with aspartate, glutamate and glycine as the predominant amino acids. Thus these low-Mr cadmium-binding proteins induced by cadmium in rat testes do not appear to be metallothionein.
J T Deagen; P D Whanger
Related Documents :
1832297 - Identification of the v1 vasopressin receptor by chemical cross-linking and ligand affi...
1520257 - Some ribosome-inactivating proteins depurinate ribosomal rna at multiple sites.
8110777 - Evidence that cyp2c19 is the major (s)-mephenytoin 4'-hydroxylase in humans.
2049077 - Heterologous expression of the allelic variant mu-class glutathione transferases mu and...
9839947 - Close vicinity of lhc b1 and lhc b4 in photosystem ii--membrane fragments as verified b...
1567207 - Selenium-dependent glutathione peroxidases from ovine and bovine erythrocytes occur as ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  231     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-12-19     Completed Date:  1985-12-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  279-83     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis
Cadmium / metabolism*
Chromatography, Gel
Chromatography, Ion Exchange
Cytosol / metabolism
Electrophoresis, Polyacrylamide Gel
Liver / metabolism
Metallothionein / isolation & purification,  metabolism*
Testis / metabolism*
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/cadmium-binding protein; 7440-43-9/Cadmium; 9038-94-2/Metallothionein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Kinetic independence of the subunits of cytosolic glutathione transferase from the rat.
Next Document:  Induction of spermidine/spermine N1-acetyltransferase in rat tissues by polyamines.