Document Detail


Properties of cadmium-binding proteins in rat testes. Characteristics unlike metallothionein.
MedLine Citation:
PMID:  4062897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since the exposure of rats to cadmium causes zinc to accumulate in metallothionein in liver and kidney but not in a similar protein in the testes, the properties of the low-Mr cadmium-binding proteins were investigated in rat testes. Weanling rats that had been given dietary cadmium for 6 weeks were injected with 109CdCl2 and subsequently killed, and the 109Cd-labelled low-Mr proteins from testes were purified. The pooled low-Mr cadmium-containing fractions from the gel-filtration (Sephadex G-75) columns were eluted through DEAE-Sephacel columns, yielding two peaks. Each of the individual peaks from this Sephacel column was further purified by rechromatography on DEAE-Sephacel and on Bio-Gel P-10 columns. Amino acid analysis of the two purified proteins revealed a low cysteine (about 3%) content, with aspartate, glutamate and glycine as the predominant amino acids. Thus these low-Mr cadmium-binding proteins induced by cadmium in rat testes do not appear to be metallothionein.
Authors:
J T Deagen; P D Whanger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  231     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-12-19     Completed Date:  1985-12-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  279-83     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Cadmium / metabolism*
Chromatography, Gel
Chromatography, Ion Exchange
Cytosol / metabolism
Electrophoresis, Polyacrylamide Gel
Liver / metabolism
Male
Metallothionein / isolation & purification,  metabolism*
Rats
Testis / metabolism*
Grant Support
ID/Acronym/Agency:
AM 19285/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/cadmium-binding protein; 7440-43-9/Cadmium; 9038-94-2/Metallothionein
Comments/Corrections

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