Document Detail

Properties and cDNA cloning of an antihemorrhagic factor (HSF) purified from the serum of Trimeresurus flavoviridis.
MedLine Citation:
PMID:  16289179     Owner:  NLM     Status:  MEDLINE    
Habu serum factor (HSF) is a metalloproteinase inhibitor that is isolated from the serum of habu snake (Trimeresurus flavoviridis), and it can suppress snake venom-induced hemorrhage. In the present study, the inhibitory property and fundamental structure of HSF were analyzed in detail. HSF inhibited all the hemorrhagic and most of the non-hemorrhagic metalloproteinases tested from the venoms of T. flavoviridis and Gloydius halys brevicaudus. HSF was extremely stable in a broad range of temperature and pH, and the treatments with a temperature of 100 degrees C or pH ranging from 1 to 13 barely affects its reactivity against G. halys brevicaudus H6 protease. Gel filtration chromatography revealed that HSF binds to the H6 protease with a 1:1 molar ratio. A secondary structure profile of HSF that was monitored by circular dichroism spectrum remained unvaried up to 2 M urea. The activity of HSF was stoichiometrically abolished by chemical modification with 2,4,6-trinitrobenzene sulfonic acid and N-bromosuccinimide; this indicates that Lys and Trp residues in its sequence play a role in the inhibitory mechanism. In this study, the amino acid sequence of HSF that was obtained by cDNA cloning was identical to that reported previously, except for five substitutions. We concluded that these discrepancies reflect a difference in the places of capture of the snake specimens.
Masanobu Deshimaru; Chie Tanaka; Kazuya Fujino; Narumi Aoki; Shigeyuki Terada; Shosaku Hattori; Motonori Ohno
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-11-10
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  46     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-02     Completed Date:  2006-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  937-45     Citation Subset:  IM    
Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Fukuoka 814-0180, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blood Proteins / genetics*,  pharmacology
Chromatography, Gel
Circular Dichroism
Cloning, Molecular
Crotalid Venoms / antagonists & inhibitors*,  chemistry*
DNA, Complementary / genetics
Hydrogen-Ion Concentration
Metalloendopeptidases / antagonists & inhibitors*
Molecular Sequence Data
Sequence Analysis, DNA
Trinitrobenzenesulfonic Acid
Reg. No./Substance:
0/Blood Proteins; 0/Crotalid Venoms; 0/DNA, Complementary; 0/Trimeresurus venoms; 0/hemorrhagic toxins (Trimeresurus); 2508-19-2/Trinitrobenzenesulfonic Acid; 39660-53-2/Bromosuccinimide; EC 3.4.24.-/Metalloendopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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