Document Detail


Properties and applications of microbial D-amino acid oxidases: current state and perspectives.
MedLine Citation:
PMID:  18084756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D: -Amino acid oxidase (DAAO) is a biotechnologically relevant enzyme that is used in a variety of applications. DAAO is a flavine adenine dinucleotide-containing flavoenzyme that catalyzes the oxidative deamination of D-isomer of uncharged aliphatic, aromatic, and polar amino acids yielding the corresponding imino acid (which hydrolyzes spontaneously to the alpha-keto acid and ammonia) and hydrogen peroxide. This enzymatic activity is produced by few bacteria and by most eukaryotic organisms. In the past few years, DAAO from mammals has been the subject of a large number of investigations, becoming a model for the dehydrogenase-oxidase class of flavoproteins. However, DAAO from microorganisms show properties that render them more suitable for the biotechnological applications, such as a high level of protein expression (as native and recombinant protein), a high turnover number, and a tight binding of the coenzyme. Some important DAAO-producing microorganisms include Trigonopsis variabilis, Rhodotorula gracilis, and Fusarium solani. The aim of this paper is to provide an overview of the main biotechnological applications of DAAO (ranging from biocatalysis to convert cephalosporin C into 7-amino cephalosporanic acid to gene therapy for tumor treatment) and to illustrate the advantages of using the microbial DAAOs, employing both the native and the improved DAAO variants obtained by enzyme engineering.
Authors:
Loredano Pollegioni; Gianluca Molla; Silvia Sacchi; Elena Rosini; Roberto Verga; Mirella S Pilone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2007-12-15
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  78     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-18     Completed Date:  2008-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  IM    
Affiliation:
Department of Biotechnology and Molecular Sciences, University of Insubria, via J.H. Dunant 3, 21100, Varese, Italy. loredano.pollegioni@uninsubria.it
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Bacteria / enzymology*
D-Amino-Acid Oxidase / genetics*,  metabolism*
Flavoproteins / chemistry,  genetics*,  metabolism*
Fungi / enzymology*
Hydrogen Peroxide / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Flavoproteins; 7722-84-1/Hydrogen Peroxide; EC 1.4.3.3/D-Amino-Acid Oxidase

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