| Properties of amino acid transport systems in K562 cells sensitive and resistant to cis-diamminedichloroplatinum(II). | |
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MedLine Citation:
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PMID: 3708576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When K562 cells were made resistant to cisplatin their neutral amino acid transport systems changed. K562 cells cloned in cisplatin developed a 6.7-fold resistance to the drug. The generation times for K562 cells sensitive (S) and resistant (DDP) to cisplatin were similar. The initial uptake and rapid efflux of cisplatin were similar in both cell lines. However, they differed in their sodium dependent neutral amino acid transport properties. In K562S cells the sodium-dependent uptake for methylaminoisobutyric acid was significantly inhibited by threonine (Ki = 10.3 mM), but in K562DDP the uptake of neutral amino acids was significantly lower, and methylaminoisobutyric acid uptake was minimally inhibited by threonine. When K562DDP cells were grown in the absence of cisplatin for 8 weeks, their amino acid transport properties reverted to those of K562S cells. In K562 cells, changes in plasma membrane essential amino acid transport systems were concomitant to both the development of resistance and the redevelopment of sensitivity to cisplatin. Therefore, human malignant cell sensitivity and resistance to cisplatin may be related to membrane nutrient transport systems. |
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Authors:
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S Shionoya; Y Lu; K J Scanlon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer research Volume: 46 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1986 Jul |
Date Detail:
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Created Date: 1986-07-23 Completed Date: 1986-07-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3445-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Biological Transport Cell Cycle / drug effects Cell Line Cisplatin Humans Sodium / metabolism Temperature |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 15663-27-1/Cisplatin; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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