Document Detail


Properties of acid beta-N-acetyl-D-glucosaminidase from cock semen.
MedLine Citation:
PMID:  1451836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two forms (I and II) of beta-N-acetyl-D-glucosaminidase from cock seminal plasma and one form (III) from spermatozoa were separated by chromatofocusing. The active enzyme forms I and II had pI values of 6.6 and 6.3, respectively, while form III had two subforms with pI values of 6.3 and 6.1, as determined by polyacrylamide gel electrofocusing. The molecular weights were 76,000 for forms I and III and 32,000 for form II. The optimum pH of enzyme forms I and III ranged from 3.6 to 4.0. In contrast, form II showed one distinct maximum at pH 3.7. The Km values obtained with p-nitrophenyl-beta-N-acetyl-D-glucosaminide as substrate were 0.35, 0.28, and 0.39 mM for forms I, II, and III, respectively. It is assumed that both cock spermatozoa and cock seminal plasma contain a common, enzymatically active beta-N-acetyl-D-glucosaminidase subunit with M(r) about 32,000 and pI 6.3.
Authors:
B Droba; M Droba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia biologica     Volume:  40     ISSN:  0015-5497     ISO Abbreviation:  Folia Biol. (Krakow)     Publication Date:  1992  
Date Detail:
Created Date:  1993-01-07     Completed Date:  1993-01-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2984758R     Medline TA:  Folia Biol (Krakow)     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  67-71     Citation Subset:  IM    
Affiliation:
Department of Basic Natural Sciences, Agricultural Academy, Rzeszów, Poland.
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MeSH Terms
Descriptor/Qualifier:
Acetylglucosaminidase / chemistry,  metabolism*
Animals
Chickens
Isoelectric Point
Isoenzymes / chemistry,  metabolism
Kinetics
Molecular Weight
Semen / enzymology*
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 3.2.1.52/Acetylglucosaminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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