Document Detail


Properties of PEI-based polyplex nanoparticles that correlate with their transfection efficacy.
MedLine Citation:
PMID:  21045811     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have evaluated the key properties of the polyethylenimine (PEI)-polyethylene glycol (PEG)-TAT peptide polyplex nanoparticles including their behavior in cells and compared them with the transfection efficacy (TE) using 11 different cell lines. We found statistically significant positive correlation between TE and the share of 50-75 nm fraction in the whole mixture of nanoparticles estimated with atomic force microscopy. Variations in PEG/PEI and N/P ratios (PEI nitrogen to DNA phosphate ratio) enabled us to find their optimal combinations, which resulted in up to 100% TE for several cell lines. Surfaces of the TE dependence of both PEG/PEI and N/P turned out to be similar in appearance for all investigated cell lines, while maximum TEs were different. We investigated subcellular transport kinetics and unpacking of the polyplex nanoparticles labeled with quantum dots (plasmid DNA) and AlexaFluor647 (block-copolymer part) using Förster Resonance Energy Transfer approach. The results demonstrated clear and statistically significant positive correlation of TE with the cellular uptake rate of the nanoparticles and negative correlation with the rate constant of their unpacking within endo/lysosomal compartments in the living cells.
Authors:
Alexey V Ulasov; Yuri V Khramtsov; Georgiy A Trusov; Andrey A Rosenkranz; Eugene D Sverdlov; Alexander S Sobolev
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-02
Journal Detail:
Title:  Molecular therapy : the journal of the American Society of Gene Therapy     Volume:  19     ISSN:  1525-0024     ISO Abbreviation:  Mol. Ther.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-04     Completed Date:  2011-04-14     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  100890581     Medline TA:  Mol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  103-12     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics of Intracellular Transport, Institute of Gene Biology, RAS, Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Cell Line, Tumor
Cyclic AMP / analogs & derivatives,  chemistry
Fluorescence Resonance Energy Transfer / methods
Gene Products, tat / chemistry
HEK293 Cells
HeLa Cells
Humans
Mice
Microscopy, Atomic Force / methods
Nanoparticles / chemistry*
Particle Size
Peptides / chemistry
Polyethylene Glycols / chemistry*,  metabolism
Polyethyleneimine / chemistry*,  metabolism
Quantum Dots
Transfection / methods*
Chemical
Reg. No./Substance:
0/Gene Products, tat; 0/Peptides; 0/Polyethylene Glycols; 39824-30-1/8-aminohexylamino cAMP; 60-92-4/Cyclic AMP; 9002-98-6/Polyethyleneimine
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