Document Detail


Properties of IgA myeloma proteins isolated rom sera of patients with the hyperviscosity syndrome.
MedLine Citation:
PMID:  404374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sera, purified myeloma proteins, and urinary proteins obtained from eight patients with igA multiple myeloma were studied by physical-chemical and immunochemical methods. In six patients whose serum viscosity was increased, the sedimentation constants of the principal component of myeloma proteins ranged from 9.1 to 10.2 S. In two patients with nearly normal serum viscosity, the sedimentation constants of these proteins were 6.2 and 7.2 S. IGA-albumin complexes were detected in most of the sera, but invarying amounts; no complexes of ig with amylase, secretory component, or alpha(1)-antitrypsin were observed. Studies on isolated myeloma proteins revealed that all igA proteisn from sera with increased viscosity represented true polymers, linked by disulfide bonds, rather than noncovalently associated aggregates; J chain was detecable by both alkaline-urea disc electrophoresis and immunoelectrophoresis with a monospecific anti-J chain serum. Increased serum viscosity was not related to the igA subclass, L chain type, or the carbohydrate compositions of individual igA myeloma proteins. The urine of five patients contained free light chains corresponding in type to the light chain of the particular igA myeloma protein. However, free J chain was not detected. The immunoelectrophoretic analysis for the presence of J chain in sera of myeloma patients may be used for early and simple detection of polymeric forms of myeloma proteins.
Authors:
J Mestecky; W J Hammack; R Kulhavy; G P Wright; M Tomana
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  89     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  919-27     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Blood Viscosity*
Chemical Phenomena
Chemistry
Female
Humans
Immunoelectrophoresis
Immunoglobulin A / analysis*
Immunoglobulin J-Chains
Immunoglobulin Light Chains
Male
Middle Aged
Molecular Conformation
Multiple Myeloma / blood*
Myeloma Proteins / analysis*,  urine
Polymers
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Immunoglobulin A; 0/Immunoglobulin J-Chains; 0/Immunoglobulin Light Chains; 0/Myeloma Proteins; 0/Polymers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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