Document Detail


Properties of D(+)-lysopine dehydrogenase from crown gall tumour tissue.
MedLine Citation:
PMID:  21695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D(+)-Lysopine dehydrogenase of an octopine-type Crown Gall tumour has been partially purified and a number of kinetic parameters have been determined. D(+)-Lysopine dehydrogenase catalyzes the reductive condensation of pyruvate and one of at least six different L-amino acids, as well as the reverse reactions, with preferential use of NADP(H) as a cofactor. The optimal pH for both reductive and oxidative reactions has been determined. At pH 6.8, L-lysine has of all the amino acids the lowest Km value, while at the same pH the highest V was found with L-arginine and L-histidine. The isoelectric point of D(+)-lysopine dehydrogenase is about 4.5.
Authors:
L A Otten; D Vreugdenhil; R A Schilperoort
Related Documents :
8012285 - 1-amino-2-imidazol-4'-ylethylphosphonic acid is a potent reversible inhibitor of pseudo...
24220995 - Neurological damage in msud: the role of oxidative stress.
31795 - Pancreatic response to intestinal perfusion with lactic acid or acidified albumin.
15545345 - Regulation of intracellular ph.
10582315 - Fatty acid composition of choline and ethanolamine glycerophospholipid subclasses in he...
12431395 - Changes in the fatty acid composition of phospholipids in tissues of farmed sea bass (d...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  485     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-01-27     Completed Date:  1978-01-27     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  268-77     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
D-Amino-Acid Oxidase / isolation & purification,  metabolism*
Kinetics
Lysine / analogs & derivatives
Plant Tumors*
Substrate Specificity
Chemical
Reg. No./Substance:
0/Amino Acids; 56-87-1/Lysine; EC 1.4.3.3/D-Amino-Acid Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of pH on the properties of normal and 5-fluorouracil-containing tRNAs.
Next Document:  Coordinate and non-coordinate accululation of aspartate transcarbamylase and dihydroorotase in synch...