| Properties of Bacteria That Trigger Hemocytopenia in the Atlantic Blue Crab, Callinectes sapidus. | |
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MedLine Citation:
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PMID: 22042435 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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In the blue crab Callinectes sapidus, injection with the bacterial pathogen Vibrio campbellii causes a decrease in oxygen consumption. Histological and physiological evidence suggests that the physical obstruction of hemolymph flow through the gill vasculature, caused by aggregations of bacteria and hemocytes, underlies the decrease in aerobic function associated with bacterial infection. We sought to elucidate the bacterial properties sufficient to induce a decrease in circulating hemocytes (hemocytopenia) as an indicator for the initiation of hemocyte aggregation and subsequent impairment of respiration. Lipopolysaccharide (LPS), the primary component of the gram-negative bacterial cell wall, is known to interact with crustacean hemocytes. Purified LPS was covalently bound to the surfaces of polystyrene beads resembling bacteria in size. Injection of these "LPS beads" caused a decrease in circulating hemocytes comparable to that seen with V. campbellii injection, while beads alone failed to do so. These data suggest that in general, gram-negative bacteria could stimulate hemocytopenia. To test this hypothesis, crabs were injected with different bacteria-seven gram-negative and one gram-positive species-and their effects on circulating hemocytes were assessed. With one exception, all gram-negative strains caused decreases in circulating hemocytes, suggesting an important role for LPS in the induction of this response. However, LPS is not necessary to provoke the immune response given that Bacillus coral, a gram-positive species that lacks LPS, caused a decrease in circulating hemocytes. These results suggest that a wide range of bacteria could impair metabolism in C. sapidus. |
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Authors:
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Nathaniel G Johnson; Louis E Burnett; Karen G Burnett |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biological bulletin Volume: 221 ISSN: 1939-8697 ISO Abbreviation: Biol. Bull. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984727R Medline TA: Biol Bull Country: United States |
Other Details:
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Languages: eng Pagination: 164-75 Citation Subset: IM |
Affiliation:
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Grice Marine Laboratory, College of Charleston, 205 Fort Johnson Rd., Charleston, South Carolina 29412, and Hollings Marine Laboratory, 331 Fort Johnson Rd, Charleston, South Carolina 29412. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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