Document Detail

Proper protein glycosylation promotes mitogen-activated protein kinase signal fidelity.
MedLine Citation:
PMID:  23210626     Owner:  NLM     Status:  MEDLINE    
The ability of cells to sense and respond appropriately to changing environmental conditions is often mediated by signal transduction pathways that employ mitogen-activated protein kinases (MAPKs). In the yeast Saccharomyces cerevisiae, the high-osmolarity glycerol (HOG) and filamentous growth (FG) pathways are activated following hyperosmotic stress and nutrient deprivation, respectively. Whereas the HOG pathway requires the MAPK Hog1, the FG pathway employs the MAPK Kss1. We conducted a comprehensive screen of nearly 5000 gene deletion strains for mutants that exhibit inappropriate cross-talk between the HOG and FG pathways. We identified two novel mutants, mnn10Δ and mnn11Δ, that allow activation of Kss1 under conditions that normally stimulate Hog1. MNN10 and MNN11 encode mannosyltransferases that are part of the N-glycosylation machinery within the Golgi apparatus; deletion of either gene results in N-glycosylated proteins that have shorter mannan chains. Deletion of the cell surface mucin Msb2 suppressed the mnn11Δ phenotype, while mutation of a single glycosylation site within Msb2 was sufficient to confer inappropriate activation of Kss1 by salt stress. These findings reveal new components of the N-glycosylation machinery needed to ensure MAPK signaling fidelity.
Evan C Lien; Michal J Nagiec; Henrik G Dohlman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-12-20
Journal Detail:
Title:  Biochemistry     Volume:  52     ISSN:  1520-4995     ISO Abbreviation:  Biochemistry     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-03-05     Revised Date:  2014-01-16    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  115-24     Citation Subset:  IM    
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MeSH Terms
Gene Deletion
Gene Expression Regulation, Fungal
Glycerol / metabolism
MAP Kinase Signaling System*
Mitogen-Activated Protein Kinases / genetics,  metabolism*
Osmotic Pressure
Saccharomyces cerevisiae / genetics,  growth & development*,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; EC Protein Kinases; PDC6A3C0OX/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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