Document Detail


Proper ascospore maturation requires the chs1+ chitin synthase gene in Schizosaccharomyces pombe.
MedLine Citation:
PMID:  10632879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have cloned chs1+, a Schizosaccharomyces pombe gene with similarity to class II chitin synthases, and have shown that it is responsible for chitin synthase activity present in cell extracts from this organism. Analysis of this activity reveals that it behaves like chitin synthases from other fungi, although with specific biochemical characteristics. Deletion or overexpression of this gene does not lead to any apparent defect during vegetative growth. In contrast, chs1+ expression increases significantly during sporulation, and this is accompanied by an increase in chitin synthase activity. In addition, spore formation is severely affected when both parental strains carry a chs1 deletion, as a result of a defect in the synthesis of the ascospore cell wall. Finally, we show that wild-type, but not chs1-/chs1-, ascospore cell walls bind wheatgerm agglutinin. Our results clearly suggest the existence of a relationship between chs1+, chitin synthesis and ascospore maturation in S. pombe.
Authors:
M Arellano; H Cartagena-Lirola; M A Nasser Hajibagheri; A Durán; M Henar Valdivieso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  35     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-03-24     Completed Date:  2000-03-24     Revised Date:  2007-08-09    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  79-89     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica, Universidad de Salamanca/CSIC, Edificio Departamental, 37007-Salamanca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chitin Synthase / genetics*
DNA Primers
Genetic Complementation Test
Microscopy, Electron
Saccharomyces cerevisiae / genetics
Schizosaccharomyces / enzymology,  genetics,  growth & development*
Spores, Fungal* / ultrastructure
Chemical
Reg. No./Substance:
0/DNA Primers; EC 2.4.1.16/Chitin Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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