Document Detail


Proparathyroid hormone: identification of a biosynthetic precursor to parathyroid hormone.
MedLine Citation:
PMID:  4501579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biosynthesis of a precursor to bovine parathyroid hormone has been demonstrated in slices of parathyroid tissue incubated in vitro. The proparathyroid hormone is 15-20 amino acids larger than the bovine hormone, and has a molecular weight of about 11,500 as determined by polyacrylamide gel electrophoresis. Upon incubation of parathyroid slices with [(14)C]aminoacids, radioactivity is detected initially in the precursor. If incorporation of [(14)C]aminoacids is inhibited after a short incubation either by replacement of radioactive amino acids with unlabeled amino acids or by addition of puromycin, the amount of radioactivity in the precursor decreases, while the radioactivity in the hormone continues to increase. The precursor is bound by an antibody that is specific for parathyroid hormone, and its binding can be inhibited by addition of the hormone. Analysis of tryptic digests indicates that the precursor and the hormone have common tryptic peptides, and that there are at least two additional peptides in the precursor.
Authors:
B Kemper; J F Habener; J T Potts; A Rich
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Mar 
Date Detail:
Created Date:  1972-05-24     Completed Date:  1972-05-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  643-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Animals
Carbon Isotopes
Cattle
Chromatography, Paper
Electrophoresis, Starch Gel
Kinetics
Parathyroid Glands / analysis,  metabolism
Parathyroid Hormone / analysis,  biosynthesis*,  metabolism
Proteins / analysis,  metabolism
Radioimmunoassay
Threonine / analysis
Tritium
Trypsin / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Parathyroid Hormone; 0/Proteins; 10028-17-8/Tritium; 72-19-5/Threonine; EC 3.4.21.4/Trypsin
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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