Document Detail


Propagation of undifferentiated human embryonic stem cells with nano-liposomal ceramide.
MedLine Citation:
PMID:  18393629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human embryonic stem (hES) cells, located on the periphery of the colonies, express the neuroectodermal markers nestin and Tuj1, suggesting a prematurely differentiated subgroup of cells. Here, we report that ceramide, a bioactive sphingolipid, selectively eliminates hES cells differentially expressing nestin and Tuj1. In contrast, undifferentiated cells are resistant to the apoptotic effects of ceramide. Ceramide-resistant hES cells express higher levels of the messenger RNA for ceramide-metabolizing enzymes that convert ceramide into pro-mitogenic metabolites. Based on these findings, we conducted long-term studies to determine whether liposomal ceramide can be used to maintain undifferentiated hES cells free of feeder cells. We continuously cultured hES cells on matrigel for 4 months with liposomal ceramide in a feeder cell-free system. Human ES cells treated with liposomal ceramide maintained their pluripotent state as determined by in vivo and in vitro differentiation studies and contained no chromosomal abnormalities. In conclusion, our findings suggest that exposure to ceramide provides a viable strategy to prevent premature hES cell differentiation and to maintain pluripotent stem cell populations in the absence of feeder cells.
Authors:
Ugur Salli; Todd E Fox; Nurgul Carkaci-Salli; Arati Sharma; Gavin P Robertson; Mark Kester; Kent E Vrana
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stem cells and development     Volume:  18     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:    2009 Jan-Feb
Date Detail:
Created Date:  2010-06-03     Completed Date:  2010-10-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-65     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Pennsylvania State University, College of Medicine, Hershey, Pennsylvania 17033-0850, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cell Culture Techniques
Cell Differentiation / physiology*
Ceramides / chemistry,  pharmacology*
Coculture Techniques
Collagen / metabolism
Drug Combinations
Embryonic Stem Cells / cytology,  drug effects*,  physiology
Humans
Intermediate Filament Proteins / metabolism
Karyotyping
Laminin / metabolism
Liposomes / chemistry*,  ultrastructure
Nanostructures / chemistry*
Nerve Tissue Proteins / metabolism
Pluripotent Stem Cells / cytology,  physiology
Proteoglycans / metabolism
Tubulin / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Ceramides; 0/Drug Combinations; 0/Intermediate Filament Proteins; 0/Laminin; 0/Liposomes; 0/Nerve Tissue Proteins; 0/Proteoglycans; 0/Tubulin; 0/beta III-tubulin protein, human; 0/nestin; 119978-18-6/matrigel; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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