Document Detail

Prooxidants prevent yeast cell death induced by genotoxic stress.
MedLine Citation:
PMID:  20942804     Owner:  NLM     Status:  MEDLINE    
It was shown earlier that DNA damage induced by alkylating agent MMS (methyl methanesulfonate) results in formation of ROS (reactive oxygen species) in yeast cells. Here, we asked whether this ROS generation is favourable for the cells. It appeared that prooxidants rather than antioxidants stimulate the survival after MMS treatment. We found that positively charged detergents increase the survival via induction of H2O2 formation in the cells. Interestingly, prooxidants protected yeast cells from the moderate doses of MMS and enhanced the toxicity of relatively high ones. Prooxidants also protect the cells arrested in mitosis (nocodazole treatment), indicating that the protection is mostly due to ROS-mediated transcriptional stress-response rather than due to enrichment of cell culture with highly MMS-resistant G2/M cells. The comparison of the published expression profile responses to prooxidant and MMS treatments identifies a set of ROS-activated genes, which are likely to protect cells from the genotoxic stress.
Dmitry A Knorre; Ekaterina A Smirnova; Olga V Markova; Maxim I Sorokin; Fedor F Severin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-11     Completed Date:  2011-07-29     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  431-5     Citation Subset:  IM    
Belozersky Institute of Physico-Chemical Biology and Institute of Mitoengineering, Moscow State University, Vorobyevy Gory 1, Moscow, Russia.
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MeSH Terms
Antioxidants / metabolism*
DNA Damage*
Methyl Methanesulfonate / pharmacology*
Mutagens / pharmacology*
Reactive Oxygen Species / metabolism*
Saccharomyces cerevisiae / cytology,  drug effects*,  metabolism
Reg. No./Substance:
0/Antioxidants; 0/Mutagens; 0/Reactive Oxygen Species; 66-27-3/Methyl Methanesulfonate

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