Document Detail


Pronounced effects of acute endurance exercise on gene expression in resting and exercising human skeletal muscle.
MedLine Citation:
PMID:  23226462     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regular physical activity positively influences whole body energy metabolism and substrate handling in exercising muscle. While it is recognized that the effects of exercise extend beyond exercising muscle, it is unclear to what extent exercise impacts non-exercising muscles. Here we investigated the effects of an acute endurance exercise bouts on gene expression in exercising and non-exercising human muscle. To that end, 12 male subjects aged 44-56 performed one hour of one-legged cycling at 50% W(max). Muscle biopsies were taken from the exercising and non-exercising leg before and immediately after exercise and analyzed by microarray. One-legged cycling raised plasma lactate, free fatty acids, cortisol, noradrenalin, and adrenalin levels. Surprisingly, acute endurance exercise not only caused pronounced gene expression changes in exercising muscle but also in non-exercising muscle. In the exercising leg the three most highly induced genes were all part of the NR4A family. Remarkably, many genes induced in non-exercising muscle were PPAR targets or related to PPAR signalling, including PDK4, ANGPTL4 and SLC22A5. Pathway analysis confirmed this finding. In conclusion, our data indicate that acute endurance exercise elicits pronounced changes in gene expression in non-exercising muscle, which are likely mediated by changes in circulating factors such as free fatty acids. The study points to a major influence of exercise beyond the contracting muscle.
Authors:
Milène Catoire; Marco Mensink; Mark V Boekschoten; Roland Hangelbroek; Michael Müller; Patrick Schrauwen; Sander Kersten
Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-30
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-28     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e51066     Citation Subset:  IM    
Affiliation:
Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Exercise / physiology*
Fatty Acids, Nonesterified / blood
Gene Expression Regulation*
Gene Regulatory Networks / genetics
Heart Rate / physiology
Humans
Hydrocortisone / blood
Insulin / blood
Leg / physiology
Male
Middle Aged
Muscle, Skeletal / metabolism*
Norepinephrine / blood
Physical Endurance / genetics*
Rest / physiology*
Transcription Factors / metabolism
Up-Regulation / genetics
Chemical
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Insulin; 0/Transcription Factors; 50-23-7/Hydrocortisone; 51-41-2/Norepinephrine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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