Document Detail


Promysalin, a Salicylate-Containing Pseudomonas putida Antibiotic, Promotes Surface Colonization and Selectively Targets Other Pseudomonas.
MedLine Citation:
PMID:  22035801     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor.
Authors:
Wen Li; Paulina Estrada-de Los Santos; Sandra Matthijs; Guan-Lin Xie; Roger Busson; Pierre Cornelis; Jef Rozenski; René De Mot
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemistry & biology     Volume:  18     ISSN:  1879-1301     ISO Abbreviation:  Chem. Biol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1320-30     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Heverlee-Leuven 3001, Belgium.
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