Document Detail

Promoter methylation and expression of Runx3 gene in gastric cancer
MedLine Citation:
PMID:  18636364     Owner:  NLM     Status:  In-Process    
OBJECTIVE: To analyze the relationships among the aberrant methylation of Runx3 gene promoter, the Runx3 protein expression and clinicopathological features in gastric cancer. METHODS: Methylation specific PCR was used to measure the promoter methylation status of Runx3 gene in tumor and the adjacent normal mucosal tissues from 40 patients with gastric cancer. Protein expression of Runx3 was measured by immunohistochemistry. RESULTS: The frequency of promoter methylaion of Runx3 gene in gastric cancer tissue(55.0%) was significantly higher compared to the adjacent normal tissues (12.5%)(P<0.01). The positive rate of protein expression of Runx3 in gastric cancer tissue(37.5%) was significantly lower compared to the adjacent normal tissues (100%). There was marked association between hypermethylaion and negative protein expression (P<0.05). The frequency of Runx3 promoter methylaion was associated with histological type, N grade, and tumor stage. CONCLUSION: The promoter hypermethylation is a main mechanism of reduced or loss expression of Runx3 gene, which may provide molecular diagnosis and stage evaluation of gastric cancer.
Li-Yi Li; Ji-Kun Li; Yun Shen; Liang Yu; Jian-Hai Zhang
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery     Volume:  11     ISSN:  1671-0274     ISO Abbreviation:  Zhonghua Wei Chang Wai Ke Za Zhi     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101177990     Medline TA:  Zhonghua Wei Chang Wai Ke Za Zhi     Country:  China    
Other Details:
Languages:  chi     Pagination:  379-82     Citation Subset:  IM    
Department of General Surgery, The Second Affiliated Hospital, Wenzhou Medical College, Wenzhou, China.
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