| Promoter hypomethylation of COMT in human placenta is not associated with the development of pre-eclampsia. | |
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MedLine Citation:
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PMID: 21047975 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Disruption of the Catechol-O-methyltransferase (COMT) gene has been shown to be involved in pre-eclampsia (PE). To investigate whether two promoters of the COMT gene are differentially regulated by methylation in PE patients, we have analyzed the genomic DNA extracted from placenta (cases n = 16; controls n = 21), maternal peripheral blood (cases n = 4; controls n = 6) and umbilical cord blood (cases n = 8; controls n = 8) of women with PE and women with normal pregnancy. Bisulfite sequencing identified the predominantly unmethylated MB-COMT promoter in placenta, maternal peripheral blood and umbilical cord blood samples (PE and control). Subsequent quantitative MassArray data confirmed a significant tissue-specific hypomethylation of the S-COMT promoter in placenta (mean = 28.6%) when compared with its densely methylated patterns in blood samples (mean = 74.5%, P < 0.001), consistent with the sequencing data. However, no PE-specific methylation difference was found between cases and controls either in placenta or in blood samples. Moreover, none of the clinical characteristics had an effect on the methylation status of the S-COMT promoter. This study does not support a causal link between methylation regulation of COMT promoters and PE. However, the observed placenta-specific S-COMT promoter may be a potential marker for early prediction of PE in maternal plasma, although this remains to be further evaluated. |
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Authors:
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Arman Zhao; Yan Cheng; Xiaotian Li; Qiaoli Li; Lei Wang; Jiawei Xu; Yuqian Xiang; Qinghe Xing; Lin He; Xinzhi Zhao |
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Publication Detail:
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Type: Journal Article Date: 2010-11-03 |
Journal Detail:
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Title: Molecular human reproduction Volume: 17 ISSN: 1460-2407 ISO Abbreviation: Mol. Hum. Reprod. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9513710 Medline TA: Mol Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 199-206 Citation Subset: IM |
Affiliation:
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Institute of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China. |
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