Document Detail

Promoter hypermethylation of a novel gene, ZHX2, in hepatocellular carcinoma.
MedLine Citation:
PMID:  16707376     Owner:  NLM     Status:  MEDLINE    
We used methylation-sensitive restriction fingerprinting (MSRF) to identify novel CpG (5'-CG-3' palindrome; p, phosphate group)-rich sequences that are methylated differentially between the hepatocellular carcinoma (HCC) genomes and adjacent nontumorous liver tissues. A 199-base-pair sequence methylated in HCC tumor tissue was isolated and showed high homology to the 5' CpG island of the zinc fingers and homeoboxes protein 2 (ZHX2) gene. By using bisulfite sequencing, we confirmed that hypermethylation of the 5' CpG island of ZHX2 occurred in some HCC and HepG2 cell lines but not in 6 normal liver tissue samples. By using methylation-specific polymerase chain reaction, we detected methylation of the 5' CpG island of ZHX2 in 46.9% of the HCCs. Reverse transcription-polymerase chain reaction demonstrated that ZHX2 messenger RNA (mRNA) was expressed in all 6 normal liver tissue samples but in only 13.3% of the methylated HCCs. Treatment of HepG2 with 5-aza-deoxycytidine could demethylate the promoter and increase ZHX2 mRNA expression. These results suggest that hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in HCC.
Zili Lv; Meng Zhang; Jiong Bi; Fangping Xu; Shaowei Hu; Jianming Wen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  125     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-18     Completed Date:  2006-05-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  740-6     Citation Subset:  AIM; IM    
Department of Pathology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
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MeSH Terms
Carcinoma, Hepatocellular / genetics,  metabolism*,  pathology
Cell Line, Tumor
DNA Methylation*
DNA, Neoplasm / analysis
Gene Expression Regulation, Neoplastic
Homeodomain Proteins / genetics,  metabolism*
Liver Neoplasms / genetics,  metabolism*,  pathology
Middle Aged
RNA, Messenger / metabolism
RNA, Neoplasm / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Transcription Factors / genetics,  metabolism*
Zinc Fingers*
Reg. No./Substance:
0/DNA, Neoplasm; 0/Homeodomain Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Transcription Factors; 0/ZHX2 protein, human

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