Document Detail


Prominent proliferative response of peripheral blood mononuclear cells to a recombinant non-structural (NS3) protein of hepatitis C virus in patients with chronic hepatitis C.
MedLine Citation:
PMID:  7544249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The proliferative response of peripheral blood mononuclear cells (PBMC) to a recombinant non-structural (NS3) protein of hepatitis C virus (HCV) was studied in 41 patients with chronic hepatitis C. Of them, 28 had chronic persistent hepatitis (CPH) and 13 chronic active hepatitis (CAH). The positive proliferation rate of PBMC to the recombinant NS3 protein, T9Ag, was 66% in the 41 patients (77% in CAH versus 61% in CPH; P > 0.05) when stimulation index (SI) = 4 was set as the cut-off value. However, mean SI of CAH patients was significantly higher than that of CPH patients (8.3 +/- 5.2 versus 5.1 +/- 3.6; P < 0.05). Six other chronic hepatitis patients who were repeatedly negative for anti-HCV antibody but positive for serum HCV RNA also had an SI of > or = 4.0. The frequency of cellular immune response to the T9Ag is among the highest results obtained by using HCV antigens tested so far. Our studies thus indicate that NS3 is an immunologically important region of HCV for T cells. Moreover, the proliferative response to T9Ag may help to establish hepatitis C etiology in chronic hepatitis patients who are seronegative with currently available anti-HCV assays.
Authors:
P M Yang; L H Hwang; M Y Lai; W L Huang; Y D Chu; W K Chi; B L Chiang; J H Kao; P J Chen; D S Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  101     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-22     Completed Date:  1995-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  272-7     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, National Taiwan University Hospital, Taipei.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Alanine Transaminase / blood
Base Sequence
Chronic Disease
DNA, Viral / genetics
Dose-Response Relationship, Drug
Female
Gene Expression
Hepacivirus / genetics,  immunology
Hepatitis Antibodies / blood
Hepatitis C / blood,  immunology*
Hepatitis C Antibodies
Humans
Leukocytes, Mononuclear / drug effects*,  immunology*
Lymphocyte Activation / drug effects*,  immunology
Male
Middle Aged
Molecular Sequence Data
Peptide Fragments / genetics,  isolation & purification,  pharmacology
Recombinant Proteins / genetics,  pharmacology
Stimulation, Chemical
T-Lymphocytes / drug effects,  immunology
Viral Nonstructural Proteins / blood,  genetics,  pharmacology*
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Hepatitis Antibodies; 0/Hepatitis C Antibodies; 0/NS3 protein, hepatitis C virus; 0/Peptide Fragments; 0/Recombinant Proteins; 0/Viral Nonstructural Proteins; EC 2.6.1.2/Alanine Transaminase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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