Document Detail

Promethazine oxidation by redox mediation in peroxidase reactions.
MedLine Citation:
PMID:  10933879     Owner:  NLM     Status:  MEDLINE    
The effect of promethazine on peroxidase-catalyzed oxidation of 3,3', 5,5'-tetramethylbenzidine was investigated at pH 5.4. Promethazine dose dependently introduced a lag in the appearance of tetramethylbenzidine charge-transfer complex monitored at 652 nm. Increasing concentrations of tetramethylbenzidine however decreased the lag period proportional to the tetramethylbenzidine concentration. Addition of promethazine to preformed charge transfer complex caused rapid bleaching of the blue-colored complex. Titration of promethazine with the yellow-colored diimine gave rise to the blue charge-transfer complex and the complete reduction of the species to the colorless parent amine compound. The available evidence suggests that promethazine is oxidized via redox mediation by tetramethylbenzidine peroxidase-oxidized products.
F J Olorunniji; S O Malomo; S A Adediran; A A Odutuga
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  380     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-12     Completed Date:  2000-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  251-6     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Biochemistry, University of Ilorin, Ilorin, P.M.B. 1515, Nigeria.
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MeSH Terms
Benzidines / metabolism
Horseradish Peroxidase / metabolism
Peroxidase / metabolism
Peroxidases / metabolism*
Promethazine / metabolism*,  pharmacology
Substrate Specificity
Reg. No./Substance:
0/Benzidines; 54827-17-7/3,3',5,5'-tetramethylbenzidine; 60-87-7/Promethazine; EC 1.11.1.-/Horseradish Peroxidase; EC 1.11.1.-/Peroxidases; EC

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