Document Detail


Prolonged stationary-phase growth of T-strain mycoplasmas in broth culture.
MedLine Citation:
PMID:  4564398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Growth of T-strain mycoplasmas in standard broth cultures has been characterized by rapid growth to peak titers of 10(6) to 10(7) color change units/ml at 20 to 24 hr, followed by a sharp decline in the viability over the next few hours. In a vacuum-flow (V-F) system utilizing negative pressure equal to 10 cm of water and air flow on the surface of broth, T strains were seen to enter the stationary phase of growth after 24 hr, and viability titers after 72 hr were >/= 10(4) color change units/ml. Maintenance of organisms in the stationary phase required both vacuum and air flow, a medium depth of 1 mm, and 1% urea in the broth. Concentration of ammonia in broth cultures under V-F remained below 200 mug/ml during 72 hr of observation. Ammonia levels in standard broth cultures exceeded 200 mug/ml after 24 hr, coincident with the decline in viability. Air passed over the medium surface in the V-F system contained large amounts of ammonia; the amount increased during each succeeding 24-hr period of observation.
Authors:
J O Hendley; E N Allred
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Infection and immunity     Volume:  5     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1972 Feb 
Date Detail:
Created Date:  1973-01-30     Completed Date:  1973-01-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  164-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agar
Ammonia / analysis,  biosynthesis
Bacteriological Techniques / instrumentation
Culture Media
Mycoplasma / growth & development*,  metabolism
Time Factors
Urea / analysis,  metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 57-13-6/Urea; 7664-41-7/Ammonia; 9002-18-0/Agar
Comments/Corrections

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