| Prolonged stationary-phase growth of T-strain mycoplasmas in broth culture. | |
| | |
MedLine Citation:
|
PMID: 4564398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Growth of T-strain mycoplasmas in standard broth cultures has been characterized by rapid growth to peak titers of 10(6) to 10(7) color change units/ml at 20 to 24 hr, followed by a sharp decline in the viability over the next few hours. In a vacuum-flow (V-F) system utilizing negative pressure equal to 10 cm of water and air flow on the surface of broth, T strains were seen to enter the stationary phase of growth after 24 hr, and viability titers after 72 hr were >/= 10(4) color change units/ml. Maintenance of organisms in the stationary phase required both vacuum and air flow, a medium depth of 1 mm, and 1% urea in the broth. Concentration of ammonia in broth cultures under V-F remained below 200 mug/ml during 72 hr of observation. Ammonia levels in standard broth cultures exceeded 200 mug/ml after 24 hr, coincident with the decline in viability. Air passed over the medium surface in the V-F system contained large amounts of ammonia; the amount increased during each succeeding 24-hr period of observation. |
| | |
Authors:
|
J O Hendley; E N Allred |
Related Documents
:
|
3040988 - Stimulation of collagenase production in human synovial fibroblast cultures by poly (i)... 6948808 - The effects of nicotine on mouse first molar tooth germs in organ culture. 1612008 - Mullerian duct regression and antiproliferative bioactivities of mullerian inhibiting s... 7489938 - Attachment of giardia lamblia trophozoites to a cultured human intestinal cell line. 12647328 - Examining culturally based variables associated with ethnicity: influences on credibili... 4963788 - Cytolytic plasma factor in experimental coccidioidomycosis. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Infection and immunity Volume: 5 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1972 Feb |
Date Detail:
|
Created Date: 1973-01-30 Completed Date: 1973-01-30 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 164-8 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Agar Ammonia / analysis, biosynthesis Bacteriological Techniques / instrumentation Culture Media Mycoplasma / growth & development*, metabolism Time Factors Urea / analysis, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 57-13-6/Urea; 7664-41-7/Ammonia; 9002-18-0/Agar |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Field studies of an attenuated Venezuelan equine encephalomyelitis vaccine (strain TC-83).
Next Document: Effect of an interferon stimulator, polyinosinic: polycytidylic acid, on experimental fungus infecti...