Document Detail


Prolonged retention of the anorectic cobalt protoporphyrin in the hypothalamus and the resulting expression of Fos.
MedLine Citation:
PMID:  15059790     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The anorectic cobalt protoporphyrin (CoPP) is known to elicit short-term hypophagia and long-term weight loss through unknown mechanisms in the brains of experimental animals. The goal of this work was to determine 1) if the prolonged duration of action of CoPP is related to its prolonged retention within the brain; and 2) with the use of immunohistochemical detection of Fos, the product of the early-immediate gene c-fos, which cells are activated after exposure to CoPP. These studies were carried out in male rats after intracerebroventricular administration of CoPP, 0.4 micromol/kg body wt, given under light halothane anesthesia. Residence of CoPP in the brain was determined by residual counts in dissected brains of (57)CoPP-injected rats. Fos immunoreactivity was mapped in coronal sections of rat brains 4-6 h after injection with CoPP. The results showed that (57)CoPP was retained in the hypothalamus preferentially compared with the cortex of the brain and could be detected in the hypothalamus for in excess of 5 wk. Fos activation was increased by CoPP, detected predominantly in neuronal rather than glial cells, and was markedly more robust in the hypothalamus than in other brain areas. Thus CoPP remains in the hypothalamus for prolonged periods and activates Fos expression in the hypothalamus.
Authors:
Richard A Galbraith; Ilean Hodgdon; Michele S Grimm; Margaret A Vizzard
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2004-04-01
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  287     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-07-23     Completed Date:  2004-08-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R465-71     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Vermont, Burlington, Vermont 05405, USA. richard.galbraith@uvm.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cobalt Radioisotopes / diagnostic use
Eating / physiology
Fluorescent Antibody Technique
Hypothalamus / metabolism*
Injections, Intraventricular
Male
Proto-Oncogene Proteins c-fos / metabolism*
Protoporphyrins / pharmacokinetics*
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
R01 DK-53479/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Cobalt Radioisotopes; 0/Proto-Oncogene Proteins c-fos; 0/Protoporphyrins; 14325-03-2/cobaltiprotoporphyrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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