| Prolonged production of hydroxyl radical in rat hippocampus after brain ischemia-reperfusion is decreased by 21-aminosteroids. | |
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MedLine Citation:
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PMID: 7824163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transient global ischemia may lead to persistent production of reactive oxygen species in selected brain regions thereby contributing to selective vulnerability to ischemia. Using cerebral microdialysis, we assessed the production of the highly reactive hydroxyl radical (OH.) in rat hippocampus during global ischemia and reperfusion (IR). During IR, perfusate containing salicylic acid was collected and analyzed for non-enzymatic hydroxylation of salicylate to 2,3-DHBA. Since 21-aminosteroids can attenuate excitatory amino acid-mediated OH. production in the brain, we repeated the experiments after administration of the 21-aminosteroid, U-74389G. The data indicate that 2,3-DHBA level increased progressively between 15 and 60 min after reperfusion, reaching values nearly three times the baseline value at 60 min. U-74389G, given 30 min before ischemia, greatly attenuated the increase in 2,3-DHBA during reperfusion. This is the first evidence for prolonged OH. production in the hippocampus after reperfusion in vivo which can be prevented by 21-aminosteroids. |
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Authors:
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J Zhang; C A Piantadosi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience letters Volume: 177 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1995-02-10 Completed Date: 1995-02-10 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 127-30 Citation Subset: IM |
Affiliation:
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Department of Medicine, Duke University Medical Center, Durham, NC 27710. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology*, therapeutic use Cerebrovascular Circulation Hippocampus / drug effects*, metabolism Hydroxybenzoic Acids / metabolism Hydroxyl Radical / metabolism* Hydroxylation / drug effects Ischemic Attack, Transient / drug therapy, metabolism* Male Pregnatrienes / pharmacology*, therapeutic use Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism Salicylic Acid Salicylic Acids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Hydroxybenzoic Acids; 0/Pregnatrienes; 0/Reactive Oxygen Species; 0/Salicylic Acids; 111668-89-4/U 74389F; 303-38-8/2-pyrocatechuic acid; 3352-57-6/Hydroxyl Radical; 69-72-7/Salicylic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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