| Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes. | |
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MedLine Citation:
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PMID: 22949030 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to investigate changes in intramuscular triglyceride (IMTG) content and perilipin 2 expression in skeletal muscle tissue following 6 mo of endurance-type exercise training in type 2 diabetes patients. Ten obese male type 2 diabetes patients (age 62 ± 1 yr, body mass index BMI 31 ± 1 kg/m²) completed three exercise sessions/week consisting of 40 min of continuous endurance-type exercise at 75% V(O₂ peak) for a period of 6 mo. Muscle biopsies collected at baseline and after 2 and 6 mo of intervention were analyzed for IMTG content and perilipin 2 expression using fiber type-specific immunofluorescence microscopy. Endurance-type exercise training reduced trunk body fat by 6 ± 2% and increased whole body oxygen uptake capacity by 13 ± 7% (P < 0.05). IMTG content increased twofold in response to the 6 mo of exercise training in both type I and type II muscle fibers (P < 0.05). A threefold increase in perilipin 2 expression was observed from baseline to 2 and 6 mo of intervention in the type I muscle fibers only (1.1 ± 0.3, 3.4 ± 0.6, and 3.6 ± 0.6% of fibers stained, respectively, P < 0.05). Exercise training induced a 1.6-fold increase in mitochondrial content after 6 mo of training in both type I and type II muscle fibers (P < 0.05). In conclusion, this is the first study to report that prolonged endurance-type exercise training increases the expression of perilipin 2 alongside increases in IMTG content in a type I muscle fiber-type specific manner in type 2 diabetes patients. |
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Authors:
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C S Shaw; S O Shepherd; A J M Wagenmakers; D Hansen; P Dendale; L J C van Loon |
Publication Detail:
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Type: Clinical Trial; Journal Article Date: 2012-09-04 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 303 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-05 Completed Date: 2013-01-07 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E1158-65 Citation Subset: IM |
Affiliation:
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School of Sport and Exercise Sciences, The University of Birmingham, Birmingham, United Kingdom. |
| Data Bank Information | |
Bank Name/Acc. No.:
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ISRCTN/ISRCTN32206301 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abdominal Fat
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pathology Adiposity Body Mass Index Cohort Studies Diabetes Mellitus, Type 2 / complications, metabolism*, pathology, therapy* Exercise* Humans Insulin Resistance Lipid Metabolism* Male Membrane Proteins / metabolism* Middle Aged Mitochondria, Muscle / metabolism, pathology Muscle Fibers, Fast-Twitch / metabolism, pathology Muscle Fibers, Slow-Twitch / metabolism*, pathology Overweight / complications Oxygen Consumption Quadriceps Muscle / metabolism*, pathology Time Factors Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins; 0/Triglycerides; 0/perilipin 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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