Document Detail


Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes.
MedLine Citation:
PMID:  22949030     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to investigate changes in intramuscular triglyceride (IMTG) content and perilipin 2 expression in skeletal muscle tissue following 6 mo of endurance-type exercise training in type 2 diabetes patients. Ten obese male type 2 diabetes patients (age 62 ± 1 yr, body mass index BMI 31 ± 1 kg/m²) completed three exercise sessions/week consisting of 40 min of continuous endurance-type exercise at 75% V(O₂ peak) for a period of 6 mo. Muscle biopsies collected at baseline and after 2 and 6 mo of intervention were analyzed for IMTG content and perilipin 2 expression using fiber type-specific immunofluorescence microscopy. Endurance-type exercise training reduced trunk body fat by 6 ± 2% and increased whole body oxygen uptake capacity by 13 ± 7% (P < 0.05). IMTG content increased twofold in response to the 6 mo of exercise training in both type I and type II muscle fibers (P < 0.05). A threefold increase in perilipin 2 expression was observed from baseline to 2 and 6 mo of intervention in the type I muscle fibers only (1.1 ± 0.3, 3.4 ± 0.6, and 3.6 ± 0.6% of fibers stained, respectively, P < 0.05). Exercise training induced a 1.6-fold increase in mitochondrial content after 6 mo of training in both type I and type II muscle fibers (P < 0.05). In conclusion, this is the first study to report that prolonged endurance-type exercise training increases the expression of perilipin 2 alongside increases in IMTG content in a type I muscle fiber-type specific manner in type 2 diabetes patients.
Authors:
C S Shaw; S O Shepherd; A J M Wagenmakers; D Hansen; P Dendale; L J C van Loon
Publication Detail:
Type:  Clinical Trial; Journal Article     Date:  2012-09-04
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  303     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-05     Completed Date:  2013-01-07     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E1158-65     Citation Subset:  IM    
Affiliation:
School of Sport and Exercise Sciences, The University of Birmingham, Birmingham, United Kingdom.
Data Bank Information
Bank Name/Acc. No.:
ISRCTN/ISRCTN32206301
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MeSH Terms
Descriptor/Qualifier:
Abdominal Fat / pathology
Adiposity
Body Mass Index
Cohort Studies
Diabetes Mellitus, Type 2 / complications,  metabolism*,  pathology,  therapy*
Exercise*
Humans
Insulin Resistance
Lipid Metabolism*
Male
Membrane Proteins / metabolism*
Middle Aged
Mitochondria, Muscle / metabolism,  pathology
Muscle Fibers, Fast-Twitch / metabolism,  pathology
Muscle Fibers, Slow-Twitch / metabolism*,  pathology
Overweight / complications
Oxygen Consumption
Quadriceps Muscle / metabolism*,  pathology
Time Factors
Triglycerides / metabolism
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Triglycerides; 0/perilipin 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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