Document Detail

Prolonged effects of acute gamma irradiation on acetylcholine-induced potassium currents in human umbilical vein endothelial cells.
MedLine Citation:
PMID:  11302774     Owner:  NLM     Status:  MEDLINE    
Bourlier, V., Diserbo, M., Gourmelon, P. and Verdetti, J. Prolonged Effects of Acute Gamma Irradiation on Acetylcholine-Induced Potassium Currents in Human Umbilical Vein Endothelial Cells. Radiat. Res. 155, 748-752 (2001). We have recently reported an acute effect of gamma irradiation (15 Gy, 1 Gy/min) on acetylcholine-mediated endothelium-dependent relaxation in rat aortic rings. Given the importance of permeability to K+ to endothelium-dependent relaxation, we have evaluated the effect of the same radiation on K+ currents in human endothelial cells in culture using the patch-clamp technique in the whole-cell recording configuration. Our results indicate that, in resting cells, gamma irradiation has no effect on endothelial permeability to K+. However, irradiation during stimulation of endothelial cells with acetylcholine reduces the sustained increase in permeability to K+ observed in the acetylcholine-stimulated, nonirradiated cells. Additional experiments using K+ channel inhibitors (TEA, charybdotoxin, apamin) suggest that irradiation may in part decrease the prolonged activation of Ca2+-activated K+ channels by acetylcholine. Taken together with our previous finding that irradiation inhibits the acute relaxing effects of acetylcholine, these results show that gamma irradiation also affects the delayed effects of acetylcholine on permeability to K+.
V Bourlier; M Diserbo; P Gourmelon; J Verdetti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  155     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-16     Completed Date:  2001-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  748-52     Citation Subset:  IM; S    
Groupe d'électrophysiologie Moléculaire, Université Joseph Fourier, BP 53X, 38041 Grenoble, France.
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MeSH Terms
Acetylcholine / pharmacology*
Calcium / metabolism
Cells, Cultured
Endothelium, Vascular / cytology,  metabolism,  radiation effects*
Gamma Rays
Potassium / metabolism*
Umbilical Veins / metabolism,  radiation effects
Reg. No./Substance:
51-84-3/Acetylcholine; 7440-09-7/Potassium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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