| Prolonged cardiac arrest and resuscitation in dogs: brain mitochondrial function with different artificial perfusion methods. | |
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MedLine Citation:
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PMID: 2984966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Clinical techniques for artificial perfusion have not previously been examined directly for their effects on brain high-energy metabolism. Our study involved 24 large mongrel dogs that were anesthetized, instrumented for central venous intravenous access, and subjected to craniotomy to expose the dura and underlying parietal cortex. The animals were divided into the following six experimental groups of four animals each: nonischemic controls; 15-minute cardiac arrest without resuscitation; 45-minute cardiac arrest without resuscitation; 15-minute cardiac arrest plus 30 minutes resuscitation with conventional cardiopulmonary resuscitation (CPR); 15-minute cardiac arrest plus 30 minutes resuscitation with interposed abdominal compression (IAC) CPR; and 15-minute cardiac arrest plus 30 minutes resuscitation with internal cardiac massage. Cardiac arrest was induced by central venous injection of KCl 0.6 mEq/kg, and it was confirmed by continuous ECG monitoring. The three active resuscitation models included administration of NaHCO3 and epinephrine, but no attempt was made to restart the heart by defibrillation during resuscitation. At the indicated time in each group, a 4- to 5-g sample of brain was removed through the craniotomy, immediately cooled to 0 C and processed for isolation of mitochondria. The mitochondria were studied for their content of superoxide dismutase and for quantitative oxygen consumption with glutamate/malate substrate during resting and ADP-stimulated respiration. Our results show a significant drop in brain mitochondrial superoxide dismutase activity during the first 15 minutes of cardiac arrest. There is minimal injury to brain mitochondrial oxygen consumption during both 15 and 45 minutes of complete ischemia.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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B C White; J F Hildebrandt; A T Evans; L Aronson; R J Indrieri; T Hoehner; L Fox; R Huang; D Johns |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of emergency medicine Volume: 14 ISSN: 0196-0644 ISO Abbreviation: Ann Emerg Med Publication Date: 1985 May |
Date Detail:
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Created Date: 1985-05-16 Completed Date: 1985-05-16 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8002646 Medline TA: Ann Emerg Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 383-8 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bicarbonates / therapeutic use Brain / metabolism* Coronary Disease / metabolism Dogs Epinephrine / therapeutic use Heart Arrest / therapy* Heart Massage Mitochondria / metabolism* Oxygen Consumption Perfusion Resuscitation / methods* Sodium Bicarbonate Superoxide Dismutase / isolation & purification Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Bicarbonates; 144-55-8/Sodium Bicarbonate; 51-43-4/Epinephrine; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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