Document Detail


Prolonged inhibition of glycogen phosphorylase in livers of Zucker Diabetic Fatty rats models human glycogen storage diseases.
MedLine Citation:
PMID:  20215584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The preclinical efficacy and safety of GPi921, a glycogen phosphorylase inhibitor, was assessed following twenty-eight days of administration to Zucker Diabetic Fatty (ZDF) rats. The ZDF rat is an animal model of type 2 diabetes mellitus (TTDM) which develops severe hyperglycemia. Inhibition of glycogen phosphorylase throughout the duration of the study was demonstrated by reductions in twenty-four-hour glucose profiles and glycated hemoglobin levels. In addition, progression towards hyperglycemia was halted in treated but not control animals, which developed hyperglycemia over the twenty-eight days of the study. Biochemical and histopathological analysis revealed large increases in hepatic glycogen, which closely paralleled the development of hepatomegaly and ultimately resulted in increases in hepatic lipids. Furthermore, prolonged glycogen phosphorylase inhibition resulted in an increased incidence and severity of other adverse pathological findings in the liver, such as inflammation, fibrosis, hemorrhage, and necrosis. The observed biochemical and histopathological phenotype of the liver closely resembled that seen in severe cases of human glycogen storage diseases (GSD) and hepatic glycogenosis in poorly controlled diabetes mellitus. These findings revealed that although glycogen phosphorylase inhibitors are efficacious agents for the control of hyperglycemia, prolonged treatment might have the potential to cause significant clinical hepatic complications that resemble those seen in GSD and hepatic glycogenosis.
Authors:
Eike Floettmann; Laraine Gregory; Joanne Teague; John Myatt; Clare Hammond; Simon M Poucher; Huw B Jones
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Publication Detail:
Type:  Journal Article     Date:  2010-03-09
Journal Detail:
Title:  Toxicologic pathology     Volume:  38     ISSN:  1533-1601     ISO Abbreviation:  Toxicol Pathol     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-30     Completed Date:  2010-08-03     Revised Date:  2012-01-05    
Medline Journal Info:
Nlm Unique ID:  7905907     Medline TA:  Toxicol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  393-401     Citation Subset:  IM    
Affiliation:
Global Safety Assessment, AstraZeneca, Alderley Park, Macclesfield, Cheshire SK10 4TG, United Kingdom. Eike.Floettmann@astrazeneca.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Area Under Curve
Blood Glucose / drug effects
Diabetes Mellitus, Type 2 / complications,  drug therapy
Disease Models, Animal
Enzyme Inhibitors / adverse effects,  pharmacokinetics
Glycogen Phosphorylase / antagonists & inhibitors*
Glycogen Storage Disease / chemically induced*,  pathology*
Humans
Hyperglycemia / drug therapy,  etiology
Hypoglycemic Agents / adverse effects*,  pharmacokinetics
Liver / drug effects*,  pathology
Male
Rats
Rats, Zucker
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Enzyme Inhibitors; 0/Hypoglycemic Agents; EC 2.4.1.-/Glycogen Phosphorylase
Comments/Corrections
Comment In:
Toxicol Pathol. 2010 Oct;38(6):1000-2   [PMID:  21037203 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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