| Proline is a protein solubilizing solute. | |
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MedLine Citation:
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PMID: 9063563 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of proline on the prevention of trichloroacetic acid (TCA)-induced protein precipitation is studied. It is found that proline at high concentrations (> 4.0 M) completely prevents TCA-induced precipitation of hen egg white lysozyme. Other osmolytes such as ethylene glycol, glycerol and sucrose fail to prevent the TCA-induced precipitation of lysozyme. Viscosity and 1-anilino-8-naphthalene sulphonic acid binding experiments suggest that proline at high concentration forms an ordered supramolecular assembly. Proline is shown to increase the solubility of protein due to formation of such higher order assemblies. A model of the supra-molecular assembly of proline is proposed and a possible in vivo role of the increased levels of proline under water stress is discussed. |
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Authors:
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D Samuel; T K Kumar; G Jayaraman; P W Yang; C Yu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and molecular biology international Volume: 41 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1997 Feb |
Date Detail:
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Created Date: 1997-06-11 Completed Date: 1997-06-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 235-42 Citation Subset: IM |
Affiliation:
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Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Egg White Female Models, Chemical Muramidase / metabolism Proline / physiology* Proteins / metabolism* Solubility Trichloroacetic Acid |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 147-85-3/Proline; 76-03-9/Trichloroacetic Acid; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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