Document Detail

Proliferative response of human endothelial cultures to various types and treatments of human sera, to culture treatments and to various oxygen concentrations.
MedLine Citation:
PMID:  7167409     Owner:  NLM     Status:  MEDLINE    
Endothelial cells from the human umbilical vein were exposed to different human sera, differently treated serum, to various oxygen concentrations, and various culture treatments. Endothelial proliferation was determined by measuring the uptake of [3H]-thymidine by means of autoradiography and presented as thymidine labeling index (TI) values. TI values differed according to different serum concentrations, serum types, serum preparations (WBS-PDS) and serum pretreatments. Low oxygen concentrations in the incubator atmosphere induced an early DNA synthesis response without an increase in cell number. The addition of the protease inhibitors aprotinin and soybean trypsin inhibitor to the culture medium resulted in a dose dependent TI decrease, whereas PMSF showed no influence.
U Schoeffel; H Maenner; J Shiga; C Mittermayer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pathology, research and practice     Volume:  175     ISSN:  0344-0338     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-05-27     Completed Date:  1983-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  348-64     Citation Subset:  IM    
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MeSH Terms
Cell Count
Cells, Cultured
Endothelium / cytology,  drug effects
Oxygen / administration & dosage,  pharmacology*
Protease Inhibitors / pharmacology
Umbilical Veins / cytology*
Reg. No./Substance:
0/Protease Inhibitors; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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