Document Detail

Proliferative inhibition, cell-cycle dysregulation, and induction of apoptosis by ursolic acid in human non-small cell lung cancer A549 cells.
MedLine Citation:
PMID:  15350828     Owner:  NLM     Status:  MEDLINE    
Ursolic acid (UA) is a pentacyclic triterpene compound isolated from many types of medicinal plants and is present in human diet. It has been reported to possess a wide range of pharmacological properties, and is one of the most promising chemopreventive agents for cancer. Here, we report that UA inhibits the cell proliferation of human lung cancer cell line A549 and provide a molecular understanding of this effect. The results showed that UA blocked cell cycle progression in the G1 phase that was associated with a marked decrease in the protein expression of cyclin D1, D2, and E and their activating partner cdk2, 4, and 6 with concomitant induction of p21/WAF1. This accumulation of p21/WAF1 might be through a p53-dependent manner. Further, UA treatment also resulted in the triggering of apoptosis as determined by DNA fragmentation assay. This effect was found to correlate with the up-regulation of Fas/APO-1, Fas ligand, and Bax, and down-regulation of NF-kappaB, Bcl-2, and Bcl-XL. Taken together, our study indicated that UA might be a potential chemopreventive agent for lung cancer.
Ya-Ling Hsu; Po-Lin Kuo; Chun-Ching Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Life sciences     Volume:  75     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-07     Completed Date:  2004-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2303-16     Citation Subset:  IM    
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, No. 100, Shin-Chuan 1st Road, 807, Taiwan.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Blotting, Western
Carcinoma, Non-Small-Cell Lung / pathology*
Cell Cycle / drug effects*
Cell Cycle Proteins / physiology
Cell Division / drug effects
Cell Line, Tumor
Enzyme-Linked Immunosorbent Assay
Fas Ligand Protein
Lung Neoplasms / pathology*
Membrane Glycoproteins / metabolism
NF-kappa B / metabolism
Signal Transduction / physiology
Triterpenes / pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Cell Cycle Proteins; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Membrane Glycoproteins; 0/NF-kappa B; 0/Triterpenes; 77-52-1/ursolic acid

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