| Proliferative effect of whey from cows' milk varying in phyto-oestrogens in human breast and prostate cancer cells. | |
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MedLine Citation:
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PMID: 22280936 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Intake of dietary phyto-oestrogens has received a great deal of attention owing to their potential influence on hormone-sensitive cancers such as breast and prostate cancer. Cows' milk contains phyto-oestrogens and the content varies according to the composition of the feed and the type and amount of legumes used. In this study we evaluated the proliferative effect of milk (whey) with different phyto-oestrogen content in human breast (MCF-7) and prostate cancer cells (PC-3). Milk was obtained from cows fed either a birdsfoot trefoil-timothy silage based ration (B1) or two different red clover silage based diets (R1 and R2) resulting in total phyto-oestrogen contents of 403, 1659 and 1434 ng/ml for the B1, R1 and R2 diets, respectively. Whey was produced from the milk and added to cell culture medium in concentrations up to 10% for MCF-7 cells and 5% for PC-3 cells. Cell proliferation was measured fluorometrically after 7 d for MCF-7 cells and 5 d for PC-3 cells. There was no significant difference in the proliferative effect of whey from the different dietary treatments at any of the whey concentrations tested. An anti-proliferative effect (P<0·01) of 5 and 10% whey was seen when tested in the presence of 10 pm oestradiol in the medium. This effect was independent of dietary treatment of cows. Whey induced a significant (P<0·01) proliferative response in PC-3 cells independent of dietary treatment. Purified equol in concentrations similar to equol concentrations in milk decreased PC-3 cell proliferation, and therefore the stimulatory effect of whey in PC-3 cells is believed to be mediated by other bioactives than equol. In conclusion, our results suggest that using whey in these proliferation assays, it was not possible to discriminate between milk with high or low levels of phyto-oestrogens. |
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Authors:
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Tina S Nielsen; Annika Höjer; Anne-Maj Gustavsson; Jens Hansen-Møller; Stig Purup |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-27 |
Journal Detail:
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Title: The Journal of dairy research Volume: - ISSN: 1469-7629 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985125R Medline TA: J Dairy Res Country: - |
Other Details:
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Languages: ENG Pagination: 1-7 Citation Subset: - |
Affiliation:
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Department of Animal Science, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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