| Proliferative effect of Apidra (insulin glulisine), a rapid-acting insulin analogue on mammary epithelial cells. | |
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MedLine Citation:
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PMID: 19480564 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The structural modification of insulin results in the generation of insulin analogues that show altered binding affinities to the insulin receptor and/or the IGF-I receptor, and as a consequence insulin analogues may have altered mitogenic potency. We analysed the proliferative effect of the rapid-acting insulin analogue Apidra (insulin glulisine) on mammary epithelial cells. We show that Apidra and Actrapid (recombinant human insulin) have similar proliferative effects on benign MCF10A and tumorigenic MCF7 cells and on epithelial cells of mouse mammary gland. Whereas Apidra and Actrapid induced similar activation of Erk1/2, activation of Akt/PKB by Apidra was significantly weaker compared to regular insulin. As AKT/PKB, an effector of the phosphoinositide 3-kinase pathway, mediates metabolic effects of insulin, we studied induction of hexokinase-2 in MCF7 cells and hexokinase-2 and hexokinase-4 in HepG2 cells by Actrapid and Apidra. Both genes were not significantly induced by Actrapid and Apidra in these cell lines. |
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Authors:
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Ashish Shukla; Harald Enzmann; Doris Mayer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of physiology and biochemistry Volume: 115 ISSN: 1744-4160 ISO Abbreviation: Arch. Physiol. Biochem. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-29 Completed Date: 2010-01-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9510153 Medline TA: Arch Physiol Biochem Country: England |
Other Details:
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Languages: eng Pagination: 119-26 Citation Subset: IM |
Affiliation:
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Hormones and Signal Transduction, German Cancer Research Centre, DKFZ-ZMBH Alliance, Heidelberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell Line Cell Proliferation / drug effects Epithelial Cells / cytology*, drug effects, pathology* Female Humans Insulin / analogs & derivatives*, genetics, metabolism, pharmacology* Mammary Glands, Animal / cytology*, drug effects Mammary Glands, Human / cytology*, drug effects Receptor, IGF Type 1 / metabolism Recombinant Proteins / metabolism, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 11061-68-0/Insulin; EC 2.7.10.1/Receptor, IGF Type 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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