Document Detail

Proliferative activity and ribosomal RNA content of 3T3 and SV40-3T3 cells.
MedLine Citation:
PMID:  6317207     Owner:  NLM     Status:  MEDLINE    
The phenomenon of resistance of confluent 3T3 cells to serum stimulation of proliferation is investigated and shown to depend drastically on the kinetics of application of growth factors with the serum. The cellular content of ribosomal RNA is determined flow-cytometrically by measuring the red fluorescence of cells stained with acridine orange. Ribosomal RNA content of 3T3 cells, but not of SV40-3T3 cells, is shown to decrease after confluence. The kinetics of restoration of ribosomal RNA in stimulated 3T3 cells exhibits an increasing delay with increasing duration of quiescence. These and further results are consistent with "progression" to initiation of the cell cycle being closely associated with restoration of cellular content of ribosomes.
G Adam; U Steiner; K Seuwen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international reports     Volume:  7     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  955-62     Citation Subset:  IM    
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MeSH Terms
Cell Division
Cell Transformation, Viral*
Cells, Cultured
RNA, Ribosomal / genetics*,  isolation & purification
RNA, Viral / genetics*,  isolation & purification
Simian virus 40 / genetics*
Reg. No./Substance:
0/RNA, Ribosomal; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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