Document Detail

Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibrous PHB scaffolds.
MedLine Citation:
PMID:  22477772     Owner:  NLM     Status:  Publisher    
This study aims at investigating the behavior in terms of the proliferation and skeletal muscle differentiation capability of two myoblastic cell lines, C2C12 and H9c2, on both isotropic and anisotropic electrospun nanofibrous poly(hydroxybutyrate) (PHB) scaffolds, as well as on PHB films and polystyrene controls. After a careful characterization of the matrices in terms of surface morphology, surface roughness and mechanical properties, the proliferation rate and the capability of the two cell lines to form skeletal myotubes were evaluated. Genetic analyses were also performed in order to assess the differentiation level of the cells on the different substrates. We demonstrated that the aligned nanofibrous mesh decreases the proliferation activity and provides a higher differentiative stimulus. We also clarified how the nanofibrous substrate influences myotube formation, and quantified a series of myotube-related parameters for both C2C12 and H9c2 cells.
Leonardo Ricotti; Alessandro Polini; Giada G Genchi; Gianni Ciofani; Donata Iandolo; Helena Vazão; Virgilio Mattoli; Lino Ferreira; Arianna Menciassi; Dario Pisignano
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-05
Journal Detail:
Title:  Biomedical materials (Bristol, England)     Volume:  7     ISSN:  1748-605X     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101285195     Medline TA:  Biomed Mater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  035010     Citation Subset:  -    
Biorobotics Institute, Polo Sant'Anna Valdera, Scuola Superiore Sant'Anna, Viale Rinaldo Piaggio 34-56025 Pontedera (Pisa), Italy. Center for MicroBioRobotics IIT@SSSA, Istituto Italiano di Tecnologia (IIT), Viale Rinaldo Piaggio 34-56025 Pontedera (Pisa), Italy.
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