Document Detail


Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.
MedLine Citation:
PMID:  8315014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.
Authors:
C H Lee; N L Bauer; M M Shaw; M M Durkin; M S Bartlett; S F Queener; J W Smith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  31     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-07-28     Completed Date:  1993-07-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1659-62     Citation Subset:  IM    
Affiliation:
Department of Pathology, Indiana University School of Medicine, Indianapolis 46202.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Line
DNA, Fungal / genetics
Embryo, Mammalian
Evaluation Studies as Topic
Female
Fibroblasts
Humans
Lung
Molecular Sequence Data
Mycology / methods*
Pneumocystis / genetics,  growth & development*,  isolation & purification
Polymerase Chain Reaction
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
N01-AI-72647/AI/NIAID NIH HHS; UO1-AI-25859-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Fungal
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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