Document Detail


Proliferation and osteogenic differentiation of rat bone marrow stromal cells on bioapatite with different crystalline facets.
MedLine Citation:
PMID:  19591234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study is to investigate the attachment, osteogenic differentiation, and collagen matrix secretion of rat bone marrow stromal cells (MSC) and MC3T3-E1 cells on the biological apatite (Ap) with different crystalline facets. The Ap samples with a-facet and c-facet surfaces were prepared by cutting the heat-treated cortical bone of bovine femurs parallel and perpendicular to the longitudinal direction, respectively. MC3T3-E1 cells attached spreading on the a-Ap sample whereas their shape of cells was round on the c-Ap. No difference in the proliferation profile of MSC and MC3T3-E1 cells was observed between the two samples. The activity of alkaline phosphatase was significantly higher for both the cells on the a-Ap sample than that of c-Ap one. The matrix of collagen secreted from MC3T3-E1 cells on the a-Ap sample oriented along the c-axis of Ap, in contrast to those of c-Ap. The cells tended to align along the collagen orientation. It is concluded that the alignment of cells is influenced by the collagen fibril orientation of Ap samples.
Authors:
Norihisa Ichinohe; Takayoshi Nakano; Toshihiro Mitaka; Yukichi Umakoshi; Yasuhiko Tabata
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  93     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  646-55     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Wiley Periodicals, Inc.
Affiliation:
Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University, South 1, West 17, Chuo-ku, Sapporo 060-8556.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Adsorption
Alkaline Phosphatase / metabolism
Animals
Apatites / chemistry*
Biocompatible Materials / chemistry
Bone Marrow Cells / cytology,  physiology*
Cattle
Cell Differentiation / physiology*
Cell Proliferation*
Cells, Cultured
Collagen / metabolism
Extracellular Matrix / chemistry,  metabolism
Fibronectins / metabolism
Materials Testing
Mice
Microscopy, Electron, Scanning
Osteocalcin / metabolism
Osteogenesis / physiology*
Rats
Rats, Inbred F344
Stromal Cells / cytology,  physiology*
Surface Properties
Vitronectin / metabolism
X-Ray Diffraction
Chemical
Reg. No./Substance:
0/Apatites; 0/Biocompatible Materials; 0/Fibronectins; 0/Vitronectin; 104982-03-8/Osteocalcin; 9007-34-5/Collagen; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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