| Proliferation and osteogenic differentiation of rat bone marrow stromal cells on bioapatite with different crystalline facets. | |
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MedLine Citation:
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PMID: 19591234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study is to investigate the attachment, osteogenic differentiation, and collagen matrix secretion of rat bone marrow stromal cells (MSC) and MC3T3-E1 cells on the biological apatite (Ap) with different crystalline facets. The Ap samples with a-facet and c-facet surfaces were prepared by cutting the heat-treated cortical bone of bovine femurs parallel and perpendicular to the longitudinal direction, respectively. MC3T3-E1 cells attached spreading on the a-Ap sample whereas their shape of cells was round on the c-Ap. No difference in the proliferation profile of MSC and MC3T3-E1 cells was observed between the two samples. The activity of alkaline phosphatase was significantly higher for both the cells on the a-Ap sample than that of c-Ap one. The matrix of collagen secreted from MC3T3-E1 cells on the a-Ap sample oriented along the c-axis of Ap, in contrast to those of c-Ap. The cells tended to align along the collagen orientation. It is concluded that the alignment of cells is influenced by the collagen fibril orientation of Ap samples. |
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Authors:
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Norihisa Ichinohe; Takayoshi Nakano; Toshihiro Mitaka; Yukichi Umakoshi; Yasuhiko Tabata |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 93 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-25 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 646-55 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Wiley Periodicals, Inc. |
Affiliation:
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Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University, South 1, West 17, Chuo-ku, Sapporo 060-8556. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Adsorption Alkaline Phosphatase / metabolism Animals Apatites / chemistry* Biocompatible Materials / chemistry Bone Marrow Cells / cytology, physiology* Cattle Cell Differentiation / physiology* Cell Proliferation* Cells, Cultured Collagen / metabolism Extracellular Matrix / chemistry, metabolism Fibronectins / metabolism Materials Testing Mice Microscopy, Electron, Scanning Osteocalcin / metabolism Osteogenesis / physiology* Rats Rats, Inbred F344 Stromal Cells / cytology, physiology* Surface Properties Vitronectin / metabolism X-Ray Diffraction |
| Chemical | |
Reg. No./Substance:
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0/Apatites; 0/Biocompatible Materials; 0/Fibronectins; 0/Vitronectin; 104982-03-8/Osteocalcin; 9007-34-5/Collagen; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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