Document Detail


Proliferation of masseter myocytes after distraction osteogenesis of the porcine mandible.
MedLine Citation:
PMID:  11243613     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Long-term success of distraction osteogenesis depends on the ability of the surrounding soft tissues to tolerate distraction forces and to adapt to the resulting increase in skeletal length and volume. The hypothesis tested in this study was that mandibular elongation by distraction induces myocyte proliferation. MATERIALS AND METHODS: Unilateral mandibular angle osteotomies were performed in 16 Yucatan minipigs. The hemimandibles were lengthened using semiburied distraction devices (Synthes Maxillofacial, Paoli, PA) with 0-day latency, 1, 2, or 4 mm/d distraction rates, and a neutral fixation period of twice the gap size in days. In 2 additional animals, the dissection and osteotomies were performed, and distraction devices were placed without activation (sham control). At the end of neutral fixation, tissues were taken from masseter muscle overlying the osteotomy and the equivalent region on the unoperated side (contralateral control). Proliferation of myocytes was estimated using immunohistochemical localization with antibodies against proliferating cell nuclear antigen (PCNA). RESULTS: Muscle overlying the distracted mandible showed 6-fold more PCNA-positive myocytes (16.8% +/- 11.3%) than the contralateral control side (2.8% +/- 1.1%, P < .0001). In the 2 sham-control animals, there was a low index of PCNA-positive myocytes on both the osteotomy (2%, 5%) and the contralateral sides (1%, 2%). CONCLUSIONS: The results of this study suggest that distraction of the porcine mandible by the protocol described induces myocyte proliferation in the masseter muscle. A proliferative response may contribute to improved long-term stability of mandibular expansion by distraction osteogenesis.
Authors:
F J Castaño; M J Troulis; J Glowacki; L B Kaban; K E Yates
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons     Volume:  59     ISSN:  0278-2391     ISO Abbreviation:  J. Oral Maxillofac. Surg.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8206428     Medline TA:  J Oral Maxillofac Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  302-7     Citation Subset:  AIM; D; IM    
Affiliation:
Skeletal Biology Research Center and Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston 02129, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Cell Nucleus / ultrastructure
Cephalometry
Female
Immunohistochemistry
Internal Fixators
Mandible / pathology,  surgery*
Masseter Muscle / pathology*
Osteogenesis, Distraction* / instrumentation,  methods
Osteotomy
Proliferating Cell Nuclear Antigen / analysis
Reproducibility of Results
Statistics, Nonparametric
Swine
Swine, Miniature
Chemical
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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