Document Detail

Proliferation kinetics of mouse tongue epithelium under normal conditions and following single dose irradiation.
MedLine Citation:
PMID:  1685035     Owner:  NLM     Status:  MEDLINE    
Epithelial proliferation in the ventral surface of mouse tongue follows a pronounced circadian rhythm with a peak in mitotic activity at 10.00 a.m., preceded by a wave of DNA synthesis 8 h earlier. Nearly all cells (85%) pass through G2 and mitosis immediately after the S-phase; they subsequently divide again, usually after 2 or 3 days, indicating cohorts of cells with different G1-duration. The fraction of all nucleated cells comprised in one daily proliferation wave is about 20%, indicating a turnover time of the nucleated cell compartment of about 5 days. Cytotoxic injury by a single radiation dose of 20 Gy causes a steep decrease in cell counts, leading to complete denudation after 9-13 days. The difference between the latent period before ulceration and the tissue turnover time is explained by a marked proliferative activity of the doomed cells. The mitotic index increases steeply after day 1 to three times the control level, but most mitotic figures display gross abnormalities such as multipolar spindles or chromosome clumping. As a consequence cells with abnormal or multiple nuclei appear in the basal layers 3 days post irradiation and subsequently migrate to the upper layers. After denudation the epithelium rapidly becomes restored, with a phase of transient hyperplasia on days 13-14. Normal architecture is regained by day 15. Over the whole healing period the mitotic index remains at a high level, with most of the mitoses appearing histologically normal.
W Dörr; J Kummermehr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virchows Archiv. B, Cell pathology including molecular pathology     Volume:  60     ISSN:  0340-6075     ISO Abbreviation:  Virchows Arch., B, Cell Pathol.     Publication Date:  1991  
Date Detail:
Created Date:  1992-02-20     Completed Date:  1992-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9316922     Medline TA:  Virchows Arch B Cell Pathol Incl Mol Pathol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  287-94     Citation Subset:  IM    
GSF--Institut für Strahlenbiologie, Neuherberg, Federal Republic of Germany.
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MeSH Terms
Cell Cycle / radiation effects
Cell Division / radiation effects
Cell Movement
Circadian Rhythm
DNA Replication / radiation effects
Epithelium / pathology,  radiation effects
Mice, Inbred C3H
Mitotic Index / radiation effects
Radiation Injuries, Experimental / pathology*
Stomatitis / etiology,  pathology*
Tongue / pathology,  radiation effects*

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