| Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique. | |
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MedLine Citation:
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PMID: 3073936 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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S-phase cells in intact animals of the coelenterate species Eirene viridula, Hydractinia echinata, Hydra attenuata, and Hydra magnipapillata incorporate the thymidine analogue bromodeoxyuridine (BrdU) into newly synthesized DNA. BrdU-labelled nuclei divide and cells appear to undergo normal differentiation. Whole-mount preparations and macerated tissues were screened for S-phase cells by means of immunocytochemical detection of BrdU (Gratzner, 1982). It is demonstrated that spatial patterns of DNA replication can be evaluated easily. Cell lineages and pathways of cell migration could be traced. |
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Authors:
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G Plickert; M Kroiher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 103 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1989-06-26 Completed Date: 1989-06-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 791-4 Citation Subset: IM |
Affiliation:
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Zoologisches Institut Universität Heidelberg, FRG. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bromodeoxyuridine Cell Differentiation* Cell Division* DNA Replication Immunohistochemistry / methods* Microscopy, Fluorescence Microscopy, Phase-Contrast |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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