| Proliferation and differentiation of neural stem cells on lysine-alanine sequential polymer substrates. | |
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MedLine Citation:
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PMID: 16516286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to explore the phenotypic potential of embryonic rat cerebral cortical stem cells by inducing differentiation on lysine-alanine sequential (LAS) polymer substrates at neurosphere level. LAS polymer is a heterologous polymer of lysine and alanine and has been demonstrated to enhance axon growth of neurons in a serum-free medium in vitro. It was found that very few cells migrated outside of the neurospheres but extremely long processes extended from differentiated cells could form a network between remote neurospheres when cells were cultured on LAS substrates at a low density of 120 neurospheres/cm(2) in the serum-free medium. On the contrary, when the neurosphere density was increased to 360 neurospheres/cm(2), many neurosphere-forming cells migrated out from their original aggregate and exhibited short processes morphology. Furthermore, when serum was added to the culture system, the neurosphere-forming cells could be induced into an extensive cellular substratum of protoplasmic cells upon which process-bearing cells spread. Clearly, neurospheres could exhibit different behaviors on LAS substrates according to the complex environmental conditions. Here, we proposed that neurospheres would change their social communication and adopt different strategies to communicate with other neurospheres when they detected each other's presence. Therefore, the mediation of cell behavior on LAS substrates by communication between neurospheres should be taken into account. |
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Authors:
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Jyh-Horng Wang; Chih-Huang Hung; Tai-Horng Young |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-03 |
Journal Detail:
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Title: Biomaterials Volume: 27 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-03-20 Completed Date: 2006-06-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 3441-50 Citation Subset: IM |
Affiliation:
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Department of Orthopedic surgery, National Taiwan University Hospital, Taipei 100, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation* Cell Movement Cell Proliferation* Cerebral Cortex / cytology, drug effects*, physiology* Embryo, Mammalian / cytology Neurons / cytology, drug effects*, physiology Phenotype Proteins / pharmacology* Rats Stem Cells / cytology, drug effects*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 0/lysine-alanine sequential polymer |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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