| Proliferation, differentiation and morphogenesis of fetal rat glandular stomach transplanted under the kidney capsule of syngeneic hosts. | |
| | |
MedLine Citation:
|
PMID: 9338599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Undifferentiated glandular stomach tissue fragments from 16.5-day fetal rats were transplanted under the kidney capsule of syngeneic adult rats, and the proliferation, differentiation and morphogenesis of the transplanted tissues were investigated. Gastric epithelial cells began to invaginate 3-4 days after the transplantation and immature glands were formed after 1 week. During the period, there was a gradual increase in the expression of pepsinogen and cathepsin E, markers of cytodifferentiation of the stomach epithelia, both at protein and mRNA levels. Cathepsin E was weakly expressed in undifferentiated gastric epithelial cells at 16.5 days of gestation, and a higher level of the expression was observed in differentiated epithelia of the transplants. In contrast, the pepsinogen-producing cells first appeared around days 3-4 after transplantation and gradually increased in number to about 30% of the epithelial cells and became localized at the bottom of the gland. During the period of the experiment up to 1 month, the pepsinogen-producing cells were all positive for class III mucin and cathepsin E, indicating the immature character of these cells. In addition, no parietal cells were observed. When the tissue fragments were transplanted into adrenalectomized animals, the epithelial differentiation and morphogenesis was suppressed, but its proliferation was enhanced. The observed changes were reversed by hydrocortisone replacement. These results suggest that the development of the 16.5-day fetal stomach is regulated intrinsically to a certain extent by the genetic program of the cells involved and various gastric functions develop in the absence of luminal stimulation, stage-specific systemic hormonal change, neuronal regulation or other systemic influences, and that glucocorticoids modulate the developmental program of the fetal stomach tissues. |
| | |
Authors:
|
M Ichinose; S Tsukada; Y Fujimitsu; M Tatematsu; Y Matsubara; N Yahagi; M Oka; T Suzuki; Y Shimizu; S Yonezawa; T Kageyama; K Miki; H Fukamachi |
Related Documents
:
|
2475529 - Lysozyme and mucins in gastric adenomas. 16603549 - Intracellular actions of group iia secreted phospholipase a2 and group iva cytosolic ph... 10913579 - Alpha-fluoromethylhistidine depletes histamine from secreting but not from non-secretin... 8025649 - Histochemical and ultrastructural analyses of glandular differentiation in typical carc... 1352909 - Somatostatin cells in the oxyntic mucosa of hypo- or hypergastrinemic rats. 1616389 - The effect of gastrin on growth of human stomach cancer cells. 14697499 - Migration patterns of subventricular zone cells in adult mice change after cerebral cor... 12591599 - Development of adrenal chromaffin cells is largely normal in mice lacking the receptor ... 17163539 - Duplication/deletion mosaicism of the 7q(21.1 --> 31.3) region. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Development, growth & differentiation Volume: 39 ISSN: 0012-1592 ISO Abbreviation: Dev. Growth Differ. Publication Date: 1997 Oct |
Date Detail:
|
Created Date: 1997-12-01 Completed Date: 1997-12-01 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0356504 Medline TA: Dev Growth Differ Country: JAPAN |
Other Details:
|
Languages: eng Pagination: 635-42 Citation Subset: IM |
Affiliation:
|
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenalectomy Animals Biological Markers / analysis Blotting, Northern Cathepsin E Cathepsins / metabolism Cell Differentiation Cell Division / physiology Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Developmental / genetics Glucocorticoids / pharmacology Histocytochemistry Kidney Morphogenesis Pepsinogens / metabolism RNA / analysis Rats Rats, Inbred F344 Stomach / cytology, embryology*, enzymology, growth & development, transplantation Transplantation, Isogeneic |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/Glucocorticoids; 0/Pepsinogens; 63231-63-0/RNA; EC 3.4.-/Cathepsins; EC 3.4.23.34/Cathepsin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Involvement of the protein of Xenopus vasa homolog (Xenopus vasa-like gene 1, XVLG1) in the differen...
Next Document: Early specification of intestinal epithelium in the chicken embryo: a study on the localization and ...