Document Detail

Proliferation and cell death of human glioblastoma cells after carbon-ion beam exposure: morphologic and morphometric analyses.
MedLine Citation:
PMID:  18282165     Owner:  NLM     Status:  MEDLINE    
Histological analyses of glioblastoma cells after carbon-ion exposure are still limited and ultrastructural characteristics have not been investigated in detail. Here we report the results of morphological and morphometric analyses of a human glioblastoma cell line, CGNH-89, after ionizing radiation to characterize the effect of a carbon-beam on glioblastoma cells. Using CGNH-89 cells exposed to 0-10 Gy of X-ray (140 kVp) or carbon-ions (18.3 MeV/nucleon, LET=108 keV/microm), we performed conventional histology and immunocytochemistry with MIB-1 antibody, transmission electron microscopy, and computer-assisted, nuclear size measurements. CGNH-89 cells with a G to A transition in codon 280 in exon 8 of the TP53 gene had nuclei with pleomorphism, marked nuclear atypia and brisk mitotic activity. After carbon-ion and X-ray exposure, living cells showed decreased cell number, nuclear condensation, increased atypical mitotic figures, and a tendency of cytoplasmic enlargement at the level of light microscopy. The deviation of the nuclear area size increased during 48 h after irradiation, while the small cell fraction increased in 336 h. In glioblastoma cells of the control, 5 Gy carbon-beam, and 10 Gy carbon-beam, and MIB-1 labeling index decreased in 24 h (12%, 11%, 7%, respectively) but increased in 48 h (10%, 20%, 21%, respectively). Ultrastructurally, cellular enlargement seemed to depend on vacuolation, swelling of mitochondria, and increase of cellular organelles, such as the cytoskeleton and secondary lysosome. We could not observe apoptotic bodies in the CGNH-89 cells under any conditions. We conclude that carbon-ion irradiation induced cell death and senescence in a glioblastoma cell line with mutant TP53. Our results indicated that the increase of large cells with enlarged and bizarre nuclei, swollen mitochondria, and secondary lysosome occurred in glioblastoma cells after carbon-beam exposure.
Takuma Oishi; Atsushi Sasaki; Nobuyuki Hamada; Shogo Ishiuchi; Tomoo Funayama; Tetsuya Sakashita; Yasuhiko Kobayashi; Takashi Nakano; Yoichi Nakazato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-16
Journal Detail:
Title:  Neuropathology : official journal of the Japanese Society of Neuropathology     Volume:  28     ISSN:  0919-6544     ISO Abbreviation:  Neuropathology     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-20     Completed Date:  2008-10-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9606526     Medline TA:  Neuropathology     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  408-16     Citation Subset:  IM    
Department of Human Pathology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
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MeSH Terms
Cell Death / radiation effects*
Cell Line, Tumor
Cell Proliferation / radiation effects
Genes, p53 / radiation effects
Glioblastoma / metabolism,  ultrastructure*
Heavy Ions*
Ki-67 Antigen / radiation effects
Linear Energy Transfer
Microscopy, Electron, Transmission
Reg. No./Substance:
0/Ki-67 Antigen; 7440-44-0/Carbon

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