| Proliferation capacity of the renal proximal tubule involves the bulk of differentiated epithelial cells. | |
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MedLine Citation:
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PMID: 17913845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated the proliferative capacity of renal proximal tubular cells in healthy rats. Previously, we observed that tubular cells originate from differentiated cells. We now found 1) by application of bromo-deoxyuridine (BrdU) for 14 days and costaining for BrdU, and the G(1)-phase marker cyclin D1 that the bulk of cells in the S3 segment of juvenile rats were involved in proliferation; 2) that although the proliferation rate was about 10-fold higher in juvenile rats compared with adult rats, roughly 40% of S3 cells were in G(1) in both groups; 3) that after a strong mitotic stimulus (lead acetate), proliferation was similar in juveniles and adults; 4) that there was a high incidence of cyclin D1-positive cells also in the healthy human kidney; and 5) by labeling dividing cells with BrdU for 2 days before the application of lead acetate and subsequent costaining for BrdU and cell cycle markers, that, although a strong mitotic stimulus does not abolish the period of quiescence following division, it shortens it markedly. Thus the capacity of the proximal tubule to rapidly recruit cells into division relies on a large reserve pool of cells in G(1) and on the shortening of the obligatory period of quiescence that follows division. |
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Authors:
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Alexander Vogetseder; Nicolas Picard; Ariana Gaspert; Michael Walch; Brigitte Kaissling; Michel Le Hir |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-03 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 294 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-01-21 Completed Date: 2008-02-21 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C22-8 Citation Subset: IM |
Affiliation:
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Institute of Anatomy, University of Zurich, Zurich, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Aging Animals Bromodeoxyuridine Cell Differentiation* / drug effects Cell Proliferation* / drug effects Cyclin D Cyclin-Dependent Kinase Inhibitor p27 / metabolism Cyclins / metabolism Epithelial Cells / drug effects, metabolism, physiology* G1 Phase Humans Ki-67 Antigen / metabolism Kidney Tubules, Proximal / drug effects, metabolism, physiology* Male Mitogens / pharmacology Organometallic Compounds / pharmacology Rats Rats, Wistar Regeneration* / drug effects Stem Cells / drug effects, metabolism, physiology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Cyclin D; 0/Cyclins; 0/Ki-67 Antigen; 0/Mitogens; 0/Organometallic Compounds; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 301-04-2/lead acetate; 59-14-3/Bromodeoxyuridine |
| Comments/Corrections | |
Comment In:
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Am J Physiol Cell Physiol. 2008 Jan;294(1):C1-3
[PMID:
17942631
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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