Document Detail


Proliferation capacity of the renal proximal tubule involves the bulk of differentiated epithelial cells.
MedLine Citation:
PMID:  17913845     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the proliferative capacity of renal proximal tubular cells in healthy rats. Previously, we observed that tubular cells originate from differentiated cells. We now found 1) by application of bromo-deoxyuridine (BrdU) for 14 days and costaining for BrdU, and the G(1)-phase marker cyclin D1 that the bulk of cells in the S3 segment of juvenile rats were involved in proliferation; 2) that although the proliferation rate was about 10-fold higher in juvenile rats compared with adult rats, roughly 40% of S3 cells were in G(1) in both groups; 3) that after a strong mitotic stimulus (lead acetate), proliferation was similar in juveniles and adults; 4) that there was a high incidence of cyclin D1-positive cells also in the healthy human kidney; and 5) by labeling dividing cells with BrdU for 2 days before the application of lead acetate and subsequent costaining for BrdU and cell cycle markers, that, although a strong mitotic stimulus does not abolish the period of quiescence following division, it shortens it markedly. Thus the capacity of the proximal tubule to rapidly recruit cells into division relies on a large reserve pool of cells in G(1) and on the shortening of the obligatory period of quiescence that follows division.
Authors:
Alexander Vogetseder; Nicolas Picard; Ariana Gaspert; Michael Walch; Brigitte Kaissling; Michel Le Hir
Related Documents :
18412175 - The application of cell-based label-free technology in drug discovery.
4509645 - Bromodeoxyuridine dependence--a new mutation in mammalian cells.
20627625 - Epoprostenol-associated pneumonitis: diagnostic use of a t-cell proliferation assay.
20722015 - Phenotypic alterations of neurons that innervate osteoarthritic joints in rats.
20086005 - Germ stem cells in the mammalian adult ovary: considerations by a fan of the primordial...
20981475 - Characterization of hybrid cells derived from spontaneous fusion events between breast ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-03
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  294     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-21     Completed Date:  2008-02-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C22-8     Citation Subset:  IM    
Affiliation:
Institute of Anatomy, University of Zurich, Zurich, Switzerland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Age Factors
Aging
Animals
Bromodeoxyuridine
Cell Differentiation* / drug effects
Cell Proliferation* / drug effects
Cyclin D
Cyclin-Dependent Kinase Inhibitor p27 / metabolism
Cyclins / metabolism
Epithelial Cells / drug effects,  metabolism,  physiology*
G1 Phase
Humans
Ki-67 Antigen / metabolism
Kidney Tubules, Proximal / drug effects,  metabolism,  physiology*
Male
Mitogens / pharmacology
Organometallic Compounds / pharmacology
Rats
Rats, Wistar
Regeneration* / drug effects
Stem Cells / drug effects,  metabolism,  physiology*
Time Factors
Chemical
Reg. No./Substance:
0/Cyclin D; 0/Cyclins; 0/Ki-67 Antigen; 0/Mitogens; 0/Organometallic Compounds; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 301-04-2/lead acetate; 59-14-3/Bromodeoxyuridine
Comments/Corrections
Comment In:
Am J Physiol Cell Physiol. 2008 Jan;294(1):C1-3   [PMID:  17942631 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Activation of ADP-ribosylation factor regulates biogenesis of the ATP7A-containing trans-Golgi netwo...
Next Document:  Dura mater-derived FGF-2 mediates mitogenic signaling in calvarial osteoblasts.