|Proliferation of airway epithelium after ozone exposure: effect of apocynin and dexamethasone.|
|PMID: 9517619 Owner: NLM Status: MEDLINE|
|Ozone is an environmental pollutant with potent oxidizing properties. We investigated whether exposure to ozone-induced cell proliferation in the lungs of rats, and determined the effect of an antioxidant and of a glucocorticosteroid in Brown-Norway (BN) rats. Following single ozone exposure (0.5, 1.0, or 3.0 ppm for 6 h), proliferating cell nuclear antigen (PCNA) expression, as determined with immunohistochemistry, was significantly increased in the bronchial epithelium and alveolar epithelium as compared with controls exposed to filtered air with a maximal effect at 24 to 48 h (p < 0.001). Apocynin (5 mg/kg, orally), a reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, reduced the PCNA index in bronchial epithelium induced by ozone (3 ppm, 6 h) from 11.5 +/- 1.3% (percent of nuclear cells expressing PCNA) to 4.4 +/- 1.3% (mean +/- SEM; p < 0.05). Dexamethasone (3 mg/kg, intraperitoneally) also reduced the PCNA index in bronchial epithelium, from 19.2 +/- 2.3% to 10.9 +/- 2.6% (p < 0.05). Dexamethasone but not apocynin inhibited ozone-induced neutrophil influx. Rats exposed repeatedly to ozone (3.0 ppm, 3 h, on three occasions 48 h apart) expressed a lower PCNA index in bronchial epithelium than did rats exposed only once at 1.9 +/- 0.7% versus 6.0 +/- 0.9%, respectively (p < 0.05). The proliferative epithelial response following a single exposure to ozone is modulated through oxidative and inflammatory mechanisms probably involving neutrophils.|
|M Salmon; H Koto; O T Lynch; E B Haddad; N J Lamb; G J Quinlan; P J Barnes; K F Chung|
Related Documents :
|23098909 - Carbachol ameliorates lipopolysaccharide-induced intestinal epithelial tight junction d...
1313399 - Mesothelioma in rats following intrapleural injection of chrysotile and phosphorylated ...
23022749 - Characterisation of spatial neglect induced by unilateral 6-ohda lesions on a choice re...
19706339 - Inhaled montelukast inhibits cysteinyl-leukotriene-induced bronchoconstriction in ovalb...
23650049 - Protective effects of carbenoxolone are associated with attenuation of oxidative stress...
23585129 - The sgc activator bay 60-2770 has potent erectile activity in the rat.
3221719 - Halothane-induced changes in contractions of mesenteric lymphatics of the rat.
19672069 - Erythropoietin sustains cognitive function and brain volume after neonatal stroke.
3088649 - A conditioned anti-parkinsonian drug effect in the hemi-parkinsonian rat.
|Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't|
|Title: American journal of respiratory and critical care medicine Volume: 157 ISSN: 1073-449X ISO Abbreviation: Am. J. Respir. Crit. Care Med. Publication Date: 1998 Mar|
|Created Date: 1998-04-07 Completed Date: 1998-04-07 Revised Date: 2006-11-15|
Medline Journal Info:
|Nlm Unique ID: 9421642 Medline TA: Am J Respir Crit Care Med Country: UNITED STATES|
|Languages: eng Pagination: 970-7 Citation Subset: AIM; IM|
|Thoracic Medicine, Imperial College School of Medicine at the National Heart and Lung Institute, London, United Kingdom.|
|APA/MLA Format Download EndNote Download BibTex|
administration & dosage,
Air Pollutants / adverse effects*
Anti-Inflammatory Agents / administration & dosage, pharmacology*
Anti-Inflammatory Agents, Non-Steroidal / administration & dosage, pharmacology*
Antioxidants / administration & dosage, pharmacology*
Bronchi / drug effects, pathology
Cell Division / drug effects
Chemotaxis, Leukocyte / drug effects
Dexamethasone / administration & dosage, pharmacology*
Enzyme Inhibitors / administration & dosage, pharmacology
Epithelium / drug effects, pathology
Gene Expression Regulation
Glucocorticoids / administration & dosage, pharmacology*
Lung / drug effects*, pathology
NADP / antagonists & inhibitors
Neutrophils / drug effects
Oxidants, Photochemical / adverse effects*
Ozone / adverse effects*
Proliferating Cell Nuclear Antigen / analysis, genetics
Pulmonary Alveoli / drug effects, pathology
Rats, Inbred BN
|0/Acetophenones; 0/Air Pollutants; 0/Anti-Inflammatory Agents; 0/Anti-Inflammatory Agents, Non-Steroidal; 0/Antioxidants; 0/Enzyme Inhibitors; 0/Glucocorticoids; 0/Oxidants, Photochemical; 0/Proliferating Cell Nuclear Antigen; 10028-15-6/Ozone; 498-02-2/acetovanillone; 50-02-2/Dexamethasone; 53-59-8/NADP|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clara cell protein (CC16) in pleural fluids: a marker of leakage through the visceral pleura.
Next Document: Risks and routes for ventilator-associated pneumonia with Pseudomonas aeruginosa.