Document Detail


Proliferation of LAMA-84 and LAMA-87 cell lines is modulated by autocrine loops involving M-CSF and TGF-beta.
MedLine Citation:
PMID:  9257809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The erythromegakaryocytic cell line (LAMA-84) and the erythroeosinophilic cell line (LAMA-87) were used to study receptor expression and receptor-mediated response to monocyte/macrophage colony-stimulating factor (M-CSF) and transforming growth factor beta (TGF-beta), two modulators of cell proliferation. As demonstrated by Northern blot analysis and reverse transcriptase polymerase chain reaction (RT-PCR), c-fms and M-CSF mRNA were expressed in both cell lines. M-CSF was detected in the supernatant of both cell lines and addition of a neutralizing anti-M-CSF antibody inhibited cell growth. The two LAMA cell lines were found to express TGF-beta1, -beta2, and -beta3 mRNAs and to secrete TGF-beta mostly in latent form. Addition of anti-TGF-beta antibodies to the culture medium increased their proliferation, whereas TGF-beta1 inhibited cell proliferation by downregulating the c-myc mRNA. These results show that the proliferation of both LAMA cell lines is positively and negatively regulated by autocrine mechanisms, implying the presence of M-CSF and TGF-beta, respectively. They suggest that similar autocrine loops could be involved in the growth regulation of leukemic cells in vivo.
Authors:
P Champelovier; P Fixe; O Valiron; J J Feige; V Praloran; D Seigneurin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental hematology     Volume:  25     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-02     Completed Date:  1997-09-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  958-65     Citation Subset:  IM    
Affiliation:
Laboratoire de Cytologie, Centre Hospitalier Régional et Universitairede Grenoble, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blast Crisis
Blotting, Northern
Cell Division*
Culture Media, Conditioned
Eosinophils* / cytology,  metabolism
Erythrocytes* / cytology,  metabolism
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Macrophage Colony-Stimulating Factor / biosynthesis,  genetics,  pharmacology*
Megakaryocytes* / cytology,  metabolism
Mice
Mice, Nude
Neoplasm Transplantation
Polymerase Chain Reaction
RNA, Messenger / metabolism
RNA-Directed DNA Polymerase
Transforming Growth Factor beta / biosynthesis,  genetics,  pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 81627-83-0/Macrophage Colony-Stimulating Factor; EC 2.7.7.49/RNA-Directed DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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