Document Detail

Proliferation of LAMA-84 and LAMA-87 cell lines is modulated by autocrine loops involving M-CSF and TGF-beta.
MedLine Citation:
PMID:  9257809     Owner:  NLM     Status:  MEDLINE    
The erythromegakaryocytic cell line (LAMA-84) and the erythroeosinophilic cell line (LAMA-87) were used to study receptor expression and receptor-mediated response to monocyte/macrophage colony-stimulating factor (M-CSF) and transforming growth factor beta (TGF-beta), two modulators of cell proliferation. As demonstrated by Northern blot analysis and reverse transcriptase polymerase chain reaction (RT-PCR), c-fms and M-CSF mRNA were expressed in both cell lines. M-CSF was detected in the supernatant of both cell lines and addition of a neutralizing anti-M-CSF antibody inhibited cell growth. The two LAMA cell lines were found to express TGF-beta1, -beta2, and -beta3 mRNAs and to secrete TGF-beta mostly in latent form. Addition of anti-TGF-beta antibodies to the culture medium increased their proliferation, whereas TGF-beta1 inhibited cell proliferation by downregulating the c-myc mRNA. These results show that the proliferation of both LAMA cell lines is positively and negatively regulated by autocrine mechanisms, implying the presence of M-CSF and TGF-beta, respectively. They suggest that similar autocrine loops could be involved in the growth regulation of leukemic cells in vivo.
P Champelovier; P Fixe; O Valiron; J J Feige; V Praloran; D Seigneurin
Related Documents :
7777359 - Inhibition of head and neck squamous cell carcinoma cell lines by transforming growth f...
19426909 - The differentiation-inducing effect of conditioned media obtained from dental pulp cells.
16601139 - Chronic palmitate but not oleate exposure induces endoplasmic reticulum stress, which m...
16537919 - Selective deletion of pten in pancreatic beta cells leads to increased islet mass and r...
12750429 - Role of cysteine s-conjugate beta-lyase in the metabolism of cisplatin.
8300609 - Secretion-capture role for apolipoprotein e in remnant lipoprotein metabolism involving...
8033899 - Generation of reactive-oxygen species induced by electropermeabilization of chinese ham...
20953859 - Resistance to platinum-based chemotherapy in lung cancer cell lines.
24875059 - Microcephaly disease gene wdr62 regulates mitotic progression of embryonic neural stem ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental hematology     Volume:  25     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-02     Completed Date:  1997-09-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  958-65     Citation Subset:  IM    
Laboratoire de Cytologie, Centre Hospitalier Régional et Universitairede Grenoble, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blast Crisis
Blotting, Northern
Cell Division*
Culture Media, Conditioned
Eosinophils* / cytology,  metabolism
Erythrocytes* / cytology,  metabolism
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Macrophage Colony-Stimulating Factor / biosynthesis,  genetics,  pharmacology*
Megakaryocytes* / cytology,  metabolism
Mice, Nude
Neoplasm Transplantation
Polymerase Chain Reaction
RNA, Messenger / metabolism
RNA-Directed DNA Polymerase
Transforming Growth Factor beta / biosynthesis,  genetics,  pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Culture Media, Conditioned; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 81627-83-0/Macrophage Colony-Stimulating Factor; EC DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Differential ability of flt3-ligand, interleukin-11, and Steel factor to support the generation of B...
Next Document:  Transduction of retrovirus-mediated NeoR gene into CD34+ cells purified from granulocyte colony-stim...