Document Detail

Proliferating resident microglia after focal cerebral ischaemia in mice.
MedLine Citation:
PMID:  17440490     Owner:  NLM     Status:  MEDLINE    
Cerebral ischaemia usually results in the rapid death of neurons within the immediate territory of the affected artery. Neuronal loss is accompanied by a sequence of events, including brain oedema, blood-brain barrier (BBB) breakdown, and neuroinflammation, all of which contribute to further neuronal death. Although the role of macrophages and mononuclear phagocytes in the expansion of ischaemic injury has been widely studied, the relative contribution of these cells, either of exogenous or intrinsic central nervous system (CNS) origin is still not entirely clear. The purpose of this study, therefore, was to use different durations of transient middle cerebral artery occlusion (tMCAo) in the mouse to investigate fully post-occlusion BBB permeability and cellular changes in the brain during the 72 h post-MCAo period. This was achieved using in vivo magnetic resonance imaging (MRI) and cell labelling techniques. Our results show that BBB breakdown and formation of the primary ischaemic damage after tMCAo is not associated with significant infiltration of neutrophils, although more are observed with longer periods of MCAo. In addition, we observe very few infiltrating exogenous macrophages over a 72 h period after 30 or 60 mins of occlusion, instead a profound increase in proliferating resident microglia cells was observed. Interestingly, the more severe injury associated with 60 mins of MCAo leads to a markedly reduced proliferation of resident microglial cells, suggesting that these cells may play a protective function, possibly through phagocytosis of infiltrating neutrophils. These data further support possible beneficial actions of microglial cells in the injured brain.
Adam Denes; Rishma Vidyasagar; Jianghua Feng; Johanna Narvainen; Barry W McColl; Risto A Kauppinen; Stuart M Allan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-18
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  27     ISSN:  0271-678X     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-16     Completed Date:  2007-12-26     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1941-53     Citation Subset:  IM    
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MeSH Terms
Antimetabolites / diagnostic use
Bromodeoxyuridine / diagnostic use
Cell Proliferation
Data Interpretation, Statistical
Ferrosoferric Oxide
Fluorescent Antibody Technique
Infarction, Middle Cerebral Artery / pathology
Ischemic Attack, Transient / pathology*
Macrophages / physiology
Magnetic Resonance Imaging
Magnetite Nanoparticles
Mice, Inbred C57BL
Microglia / pathology*
Monocytes / physiology
Neutrophil Infiltration
Phagocytes / physiology
Grant Support
G9219675//Medical Research Council
Reg. No./Substance:
0/Antimetabolites; 0/Magnetite Nanoparticles; 0/Oxides; 0/ferumoxtran-10; E1UOL152H7/Iron; G34N38R2N1/Bromodeoxyuridine; K3R6ZDH4DU/Dextrans; XM0M87F357/Ferrosoferric Oxide

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